中文摘要：病毒编码沉默抑制蛋白以抵消RNA沉默。由于dsRNA在沉默中起着关键的作用，常规的沉默抑制策略是双链RNA的结合。植物病毒番茄褪绿病毒（ToCV）的抑制子p22已经被认为是具有局部抑制活性持续时间最长的抑制子之一。然而，p22的作用机制尚未有研究。本研究发现：ToCV p22与长dsRNAs体外结合，从而干扰长dsRNAs通过RNase III型Dicer同源酶变成小RNAs (sRNAs)。
外文摘要：Viruses encode silencing suppressor proteins to counteract RNA silencing. Because dsRNA plays a key role in silencing, a general silencing suppressor strategy is dsRNA binding. The p22 suppressor of the plant virus Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) has been described as having one of the longest lasting local suppressor activities. However, the mechanism of action of p22 has not been characterized. Here, we show that ToCV p22 binds long dsRNAs in vitro, thus interfering with their processing into small RNAs (sRNAs) by an RNase III-type Dicer homolog enzyme. Additionally, we have studied whether a putative zinc finger motif found in p22 has a role in dsRNA binding and suppressor function. The efficient ability of p22 to suppress RNA silencing, triggered by hairpin transcripts transiently expressed in planta, supports the relationship between its ability to bind dsRNA in vitro and its ability to inhibit RNA silencing in vivo.
外文关键词：Closteroviridae; Crinivirus; Tomato chlorosis virus; RNA silencing suppressor; RNA binding protein
作者：Landeo-Rios, Yazmin; Navas-Castillo, Jesus; Moriones, Enrique; 等
作者单位：马拉加大学
期刊名称：VIROLOGY
期刊影响因子：3.2
出版年份：2016
出版刊次：1
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