中文摘要：对病毒耐受的植物是揭示vsRNAs抗病毒效率的有用材料，可允许病毒积累，然后产生病毒衍生的小RNAs（vsRNAs）。本研究通过对番茄黄化曲叶病毒耐受和敏感的番茄品种中vsRNAs的比较，揭示了vsRNAs在病毒基因组上一致的分布趋势，该分布趋势被认为是一个明显的特征。
外文摘要：Virus-tolerant plant, which allows the accumulation of virus and then generates virus-derived small RNAs (vsRNAs), is a valuable material to reveal the antiviral efficiency of vsRNAs. Here, a comparison of vsRNAs in Tomato yellow leaf curl virus tolerant and in susceptible tomato varieties showed the consistent trend of vsRNAs' distribution on virus genome, which is presented as an obvious characteristic. However, the expression level of vsRNA in tolerant variety is less than that in susceptible variety. Slicing targets of vsRNA-mediated viral transcripts were investigated using parallel analysis of RNA ends, and geminivirus DNA methylation was determined by bisulfite sequencing, which uncovered that not all vsRNAs participated in viral mRNA degradation and DNA methylation. Additionally, by comparing with the expression pattern of vsRNAs, viral DNA and mRNA, we proposed the quantity of vsRNAs is corresponding to the expression level of viral mRNA, while the virus-suppression of vsRNAs is not high-efficient.
外文关键词：Tomato yellow leaf curl virus; virus-induced RNA silencing; virus-derived small RNA; degradome
作者：Bai Miao; Yang Guo-shun; Chen Wen-ting; 等
作者单位：中国农业科学院
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE
期刊影响因子：0.724
出版年份：2016
出版刊次：2
点击下载：耐受与敏感番茄品种中番茄黄化曲叶病毒衍生的小RNAs的特征与功能研究