中文摘要：RNA依赖的RNA聚合酶（RDRs）在基因沉默中起着关键作用。分析发现，水稻OsRDR6突变体shl2-rol接种黄瓜花叶病毒、水稻坏死花叶病毒、水稻白叶枯病菌或稻瘟病菌后，水稻RDR6基因对病毒、细菌和真菌病原体有响应。与野生型相比，突变株接种病毒后积累了较多的病毒RNA，并在接种细菌或真菌后产生了较严重的症状。因此，OsRDR6介导的RNA沉默路径可能不仅参与了对病毒的防御，还参与了对细菌与真菌的抵抗。
外文摘要：RNA-dependent RNA polymerases (RDRs) play key roles in gene silencing. The rice RDR6 gene was analyzed in response to viral, bacterial and fungal pathogens, after inoculation of a rice mutant line of OsRDR6, shl2-rol, with Cucumber mosaic virus, Rice necrosis mosaic virus, Xanthomonas oryzae pv. oryzae or Magnaporthe oryzae. Compared with the wild type, the mutant line accumulated more viral RNA after inoculation with the viruses and developed more severe symptoms after inoculation with the bacterium or fungus. Thus, the OsRDR6-mediated RNA silencing pathway seems to participate in defense against not only viruses, but also bacterial and fungal pathogens.
外文关键词：OsRDR6; shl2-rol; Rice; Cucumber mosaic virus; Rice necrosis mosaic virus; Xanthomonas oryzae pv. oryzae; Magnaporthe oryzae
作者：Wagh, S. G.; Alam, M. M.; Kobayashi, K.; 等
作者单位：日本爱媛大学
期刊名称：JOURNAL OF GENERAL PLANT PATHOLOGY
期刊影响因子：1.12
出版年份：2016
出版刊次：1
点击下载：响应病毒、细菌和真菌病原体的水稻RNA依赖的RNA聚合酶6（OsRDR6）基因分析