中文摘要：很多短干扰RNAs（siRNAs）是在植物病毒侵染的过程中产生的，但他们的功能、特征和发生尚不清楚。本文利用高通量深度测序技术分析了黄瓜花叶病毒（CMV）Fny胁迫影响下番茄植株中病毒小RNA的功能。研究结果表明：病毒来源的siRNAs的产生可能参与了病毒-寄主之间的交互作用，并有助于解释病原体如何引起寄主症状。
外文摘要：A Large number of short interfering RNAs (siRNAs) are generated from plant viruses during infection, but their function, character and biogenesis are unclear. Here, we analyzed the viral small RNA profile in tomato plants upon Cucumber mosaic virus (CMV) Fny strain infection, using high-throughput deep sequencing techniques. A set of 129666 CMV-Fny-specific small RNAs, most of the 21-(54.6%) and 22-nucleotide (28.2%) in size were obtained. Both strands of CMV-Fny were substrates for production of viral-specific siRNAs, and the virus genome harbored multiple, highly reproducible small RNA-generating hot spots that corresponded to regions with no apparent local hairpin-forming capacity. In addition, using the novel high throughput degradom sequencing approach and informatics analysis, we found a large set of host genes could be potentially targeted by CMV-Fny-derived siRNAs for posttranscriptional silencing. Six of such host targets were further validated by qRT-PCR. Gene ontology analysis with biological process, cellular component and molecular function terms revealed that these target genes mainly related to stress response, cell redox homeostasis, protein folding/stabilization/transport, chloroplast-and photosynthesis-related processes, metal ion binding, ATP binding, jasmonic acid mediated signaling pathway, and gene silencing by RNA. These results indicate that the production of viral derived siRNAs might be involved in virus-host interactions, and could contribute to explain how pathogens cause symptoms in their hosts.
外文关键词：Cucumber Mosaic Virus; Short Interfering RNA; Genome; High-Throughput Deep Sequencing; Degradom Analysis
作者：Feng, Junli; Liu, Shasha; Chen, Jishuang
作者单位：浙江工商大学
期刊名称：JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
期刊影响因子：1.338
出版年份：2016
出版刊次：7
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