中文摘要：由烟粉虱传播的番茄黄化曲叶病毒（TYLCV）已对植物生长和发展构成了严重的威胁。植物抵抗各种威胁的先天免疫系统包括WRKY转录因子（TFs）家族III。TFs是参与植物生物进程的一个主要的成分。本文研究鉴定了6个WRKY家族III转录因子（SolyWRKY41、SolyWRKY42、SolyWRKY53、 SolyWRKY54、SolyWRKY80和SolyWRKY81），这些转录因子均对TYLCV侵染有反应。
外文摘要：Background: Transmitted by the whitefly Bemisia tabaci, tomato yellow leaf curly virus (TYLCV) has posed serious threats to plant growth and development. Plant innate immune systems against various threats involve WRKY Group III transcription factors (TFs). This group participates as a major component of biological processes in plants. Results: In this study, 6 WRKY Group III TFs (SolyWRKY41, SolyWRKY42, SolyWRKY53, SolyWRKY54, SolyWRKY80, and SolyWRKY81) were identified, and these TFs responded to TYLCV infection. Subcellular localization analysis indicated that SolyWRKY41 and SolyWRKY54 were nuclear proteins in vivo. Many elements, including W-box, were found in the promoter region of Group III TFs. Interaction network analysis revealed that Group III TFs could interact with other proteins, such as mitogen-activated protein kinase 5 (MAPK) and isochorismate synthase (ICS), to respond to biotic and abiotic stresses. Positive and negative expression patterns showed that WRKY Group III genes could also respond to TYLCV infection in tomato. The DNA content of TYLCV resistant lines after SolyWRKY41 and SolyWRKY54 were subjected to virus-induced gene silencing (VIGS) was lower than that of the control lines. Conclusions: In the present study, 6 WRKY Group III TFs in tomato were identified to respond to TYLCV infection. Quantitative real-time-polymerase chain reaction (RT-qPCR) and VIGS analyses demonstrated that Group III genes served as positive and negative regulators in tomato-TYLCV interaction. WRKY Group III TFs could interact with other proteins by binding to cis elements existing in the promoter regions of other genes to regulate pathogenrelated gene expression.
外文关键词：TYLCV; WRKY Group III TFs; VIGS; Regulation mechanism; Tomato
作者：Huang, Ying; Li, Meng-Yao; Wu, Peng; 等
作者单位：南京农业大学
期刊名称：BMC GENOMICS
期刊影响因子：3.867
出版年份：2016
出版刊次：10
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