中文摘要：番茄黄化曲叶病毒（TYLCV）是一种菜豆金色花叶病毒属病毒，是全球栽培番茄上最重要的病毒之一，主要会引起植株叶片黄化、卷曲和矮化。TYLCV在亚热带和热带国家以及韩国引起了严重的问题。虽然每个病毒成分的功能已被确定，然而，TYLCV侵染机制仍然未知。本研究结果表明：TYLCV C4 可能有助于抑制TYLCV侵染时寄主的抗性，并参与了病毒感染的泛素化。
外文摘要：Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, is one of the most important viruses of cultivated tomatoes worldwide, mainly causing yellowing and curling of leaves with stunting in plants. TYLCV causes severe problems in sub-tropical and tropical countries, as well as in Korea. However, the mechanism of TYLCV infection remains unclear, although the function of each viral component has been identified. TYLCV C4 codes for a small protein involved in various cellular functions, including symptom determination, gene silencing, viral movement, and induction of the plant defense response. In this study, through yeast-two hybrid screenings, we identified TYLCV C4-interacting host proteins from both healthy and symptom-exhibiting tomato tissues, to determine the role of TYLCV C4 proteins in the infection processes. Comparative analyses of 28 proteins from healthy tissues and 36 from infected tissues showing interactions with TYLCV C4 indicated that TYLCV C4 mainly interacts with host proteins involved in translation, ubiquitination, and plant defense, and most interacting proteins differed between the two tissues but belong to similar molecular functional categories. Four proteins two ribosomal proteins, S-adenosyl-L-homocysteine hydrolase, and 14-3-3 family protein were detected in both tissues. Furthermore, the identified proteins in symptom-exhibiting tissues showed greater involvement in plant defenses. Some are key regulators, such as receptor-like kinases and pathogenesis-related proteins, of plant defenses. Thus, TYLCV C4 may contribute to the suppression of host defense during TYLCV infection and be involved in ubiquitination for viral infection.
外文关键词：defense genes; protein-protein interactions; tomato; Tomato yellow leaf curl virus; yeast-two hybrid
作者：Kim, Namgyu; Kim, Jinnyun; Bang, Bongjun; 等
作者单位：韩国釜山国立大学
期刊名称：PLANT PATHOLOGY JOURNAL
期刊影响因子：0.92
出版年份：2016
出版刊次：10
点击下载：健康番茄和感染病毒番茄组织中番茄黄化曲叶病毒C4蛋白交互影响寄主蛋白的比较研究