中文摘要：百合是最重要的花卉作物之一，为了加快百合遗传学研究，开发反向遗传工具是必要的。然而，百合中农杆菌介导的转化比较耗时，因为植物需要好几年才能从驯化发展到开花。因此，病毒诱导的基因沉默（VIGS）是分析基因功能的一种很有吸引力的工具。本研究结果表明，CMV-HL侵染的第一年在百合的叶片和花上系统性传播，次年传播到新梢上，并且载体系统可成功应用于诱导百合短期的内源基因沉默。
外文摘要：Lilies (Lilium) are among the most important floriculture crops, and to accelerate research regarding lily genetics, the development of reverse-genetics tools is necessary. However, Agrobacterium-mediated transformation in Lilium is time-consuming, since the plants require several years to progress from acclimation to flowering. Thus, virus-induced gene silencing (VIGS) is an attractive method for assaying gene function. In the present study, we modified a lily-derived strain of Cucumber mosaic virus (CMV-HL) as a VIGS vector and evaluated its effectiveness for inducing gene silencing in Lilium leichtlinii by introducing L. leichtlinii phytoene desaturase (LlPDS) gene fragments into an intercistronic region between the 3a and 3b genes of the CMV-HL RNA3 genome. At 30 days after inoculation (dpi) with LlPDS-containing CMV-HL, photo-bleaching was observed in the upper leaves of L. leichtlinii, and at 57 dpi, we observed that the natural orange color in flower tepals had faded. Reduced LlPDS expression and the detection of small interfering LlPDS RNA indicated that the color changes were the result of LlPDS gene silencing. In addition, the leaves also exhibited a mild photo-bleaching phenotype in the following year. Therefore, our results indicate that CMV-HL spreads systemically in the leaves and flowers of Lilium during the first year of infection, as well as in new shoots during the following year, and that the vector system can be successfully applied to induce short-term endogenous gene silencing in lilies.
外文关键词：Cucumber mosaic virus (CMV)-HL; lily; phytoene desaturase (PDS); photo-bleaching; small interfering RNA (siRNA)
作者：Tasaki, Keisuke; Terada, Hiroyuki; Masuta, Chikara; 等
作者单位：日本北海道大学
期刊名称：PLANT BIOTECHNOLOGY
期刊影响因子：0.715
出版年份：2016
出版刊次：12
点击下载：百合属植物中使用黄瓜花叶病毒载体进行的病毒诱导的基因沉默（VIGS）