中文摘要：番茄黄化曲叶病毒（TYLCV）是一种由烟粉虱传播的菜豆金黄花叶病毒属病毒。病毒在昆虫体内循环移位是众所周知的。但是，对TYLCV从消化道进入血淋巴却知之甚少。本研究表明，网格蛋白介导的内吞作用和核内体在TYLCV转运至粉虱中肠中起到了重要的作用。
外文摘要：Tomato yellow leaf curl virus (TYLCV) is a begomovirus transmitted by the whitefly Bemisia tabaci. The circulative translocation of the virus in the insect is known in its broad line. However, transit of TYLCV from the digestive tract into the haemolymph is poorly understood. We studied the involvement of clathrin in this process by disrupting the clathrin-mediated endocytosis and the endosome network using inhibitor feeding, antibody blocking and dsRNA silencing. We monitored the quantities of TYLCV in the whitefly and virus transmission efficiency. Following endocytosis and endosome network disruption, the quantity of virus was higher in the midgut relative to that of the whole insect body, and the quantity of virus in the haemolymph was reduced. The transmission efficiency of TYLCV by the treated insects was also reduced. These findings indicate that clathrin-mediated endocytosis and endosomes play an important role in the transport of TYLCV across the whitey midgut.
外文关键词：Bemisia tabaci; Clathrin-mediated endocytosis; Digestive tract; Tomato yellow leaf curl virus; Virus transmission
作者：Pan, Li-Long; Chen, Qun-Fang; Zhao, Juan-Juan; 等
作者单位：浙江大学
期刊名称：VIROLOGY
期刊影响因子：3.2
出版年份：2017
出版刊次：2
点击下载：网格蛋白介导的内吞作用在番茄黄化曲叶病毒穿过粉虱介体中肠传播中的作用研究