中文摘要：南方菜豆花叶病毒组P1蛋白（之前以转录后基因沉默的抑制子为特征）是病毒在植物中系统传播与侵染所必需的。本研究发现，水稻黄斑驳病毒（RYMV）在测试的寄主或非寄主植物种类中复制和局部移动时不需要P1。然而，它对病毒在寄主植物水稻上成功的系统传播很重要。
外文摘要：Sobemovirus P1 protein, characterized previously as a suppressor of posttranscriptional gene silencing, is required for systemic virus spread and infection in plants. Mutations in the ORF1 initiation codon do not affect viral replication indicating P1 is not necessary for this process. Wild type, recombinant and P1 deletion mutants of Cocksfoot mottle virus and Rice yellow mottle virus were used to infect oat, rice, wheat, barley, Arabidopsis thaliana and Nicotiana benthamiana plants. Wild type RYMV, RYMV without P1 and RYMV with CfMV P1 were detected in inoculated leaves of all tested plant species. We found that RYMV does not need P1 for replication and for local movement neither in host nor non-host species tested in this study. However, it is crucial for successful systemic spread of the virus in its host plant rice. Moreover, adding CfMV P1 into RYMV genome did not help it to overcome restriction to the inoculated leaf.
外文关键词：Sobemovirus; Virus replication; P1 gene; Local movement; Oat; Wheat; Barley; Rice; Nicotiana benthamiana; Arabidopsis thaliana
作者：Nummert, Grete; Somera, Merike; Uffert, Gabriela; 等
作者单位：爱沙尼亚塔林理工大学
期刊名称：VIROLOGY
期刊影响因子：3.2
出版年份：2017
出版刊次：2
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