中文摘要：双生病毒是植物病毒种最大和最具破坏性的病毒组，含有ssDNA作为遗传物质。双生病毒衍生病毒诱导的基因沉默（VIGS）载体已成为研究各种植物功能基因组学的一种简单且有效的工具。然而，先前开发的VIGS载体具有某些局限性，不能用于组织特异性功能的研究。本文研究开发了一个基于辣椒卷叶病毒(ChiLCV)的VIGS载体，该载体提供了一个有效且灵活的工具，可用于揭示某些韧皮部特异基因的未知功能。
外文摘要：Geminiviruses are the largest and most devastating group of plant viruses which contain ssDNA as a genetic material. Geminivirus-derived virus-induced gene silencing (VIGS) vectors have emerged as an efficient and simple tool to study functional genomics in various plants. However, previously developed VIGS vectors have certain limitations, owing to their inability to be used in tissue-specific functional study. In the present study, we developed a Chilli leaf curl virus (ChiLCV)-based VIGS vector for its tissue-specific utilization by replacing the coat protein gene (open reading frame (ORF) AV1) with the gene of interest for phytoene desaturase (PDS) of Nicotiana benthamiana. Functional validation of ChiLCV-based VIGS in N. benthamiana resulted in systemic silencing of PDS exclusively in the phloem region of inoculated plants. Furthermore, expression of enhanced green fluorescence protein (EGFP) using the same ChiLCV vector was verified in the phloem region of the inoculated plants. Our results also suggested that, during the early phase of infection, ChiLCV was associated with the phloem region, but at later stage of pathogenesis, it can spread into the adjoining non-vascular tissues. Taken together, the newly developed ChiLCV-based vector provides an efficient and versatile tool, which can be exploited to unveil the unknown functions of several phloem-specific genes.
外文关键词：ChiLCV; Geminivirus; VIGS vector; Phloem specific; Virus movement; Expression vector
作者：Kushwaha, Nirbhay Kumar; Chakraborty, Supriya
作者单位：贾瓦哈拉尔尼赫鲁大学
期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
期刊影响因子：3.376
出版年份：2017
出版刊次：3
点击下载：一种基于辣椒卷叶病毒的用于研究韧皮部特异外源基因沉默以及外源基因过表达的载体研发