中文摘要：如今面临着这样的一个风险，即番茄黄化曲叶病毒（TYLCV）与其介体烟粉虱将出现在世界温带地区的温室大棚里，包括北欧。本文通过农杆菌介导的传染性克隆和滚环扩增法产物的基因递送，证明了爱沙尼亚进口番茄果实中分离出来的TYLCV 可感染番茄植株和烟草。从番茄果实扩增的TYLCV基因组具有传染性，这证实了番茄果实可以作为病毒感染源。
外文摘要：There is a risk that Tomato yellow leaf curl virus (TYLCV) and its vector, whiteflies of the Bemisia tabaci species complex, will become established in greenhouses in temperate regions of the world, including northern Europe. In this study, TYLCV isolated from imported tomato fruit in Estonia (originating from Spain) was shown to be able to infect plants of tomato and Nicotiana benthamiana using Agrobacterium-mediated inoculation with an infectious clone as well as using biolistic delivery of products from rolling circle amplification (RCA). A 1.8-mer genomic construct of TYLCV was engineered and efficiently agroinfiltrated into plants of tomato and N. benthamiana, and induced symptoms characteristic of natural infection. With Agrobacterium-mediated inoculation, the infection efficiency was 100% for both tomato and N. benthamiana, whereas biolistic inoculation using RCA products resulted in efficiencies of 57% and 36%, respectively. Particle bombardment with monomeric linear genome failed to produce any infection in tomato or N. benthamiana. The genome of TYLCV amplified from tomato fruit was infectious confirming that tomato fruit may serve as a source of virus inoculum. This is the first report of agroinfiltration and particle bombardment assay using TYLCV DNA derived from infected tomato fruit tissue.
外文关键词：agroinfiltration; begomovirus; infectious clone; Solanum lycopersicum; tomato fruit
作者：Just, Kadri; Sattar, Muhammad Naeem; Arif, Usman; 等
作者单位：爱沙尼亚大学
期刊名称：ZEMDIRBYSTE-AGRICULTURE
期刊影响因子：0.579
出版年份：2017
出版刊次：3
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