中文摘要：最近有些研究已报道了促有丝分裂蛋白激酶（MAPK3）在植物免疫反应中的作用。然而，受番茄黄化曲叶病毒侵染后番茄体内MAPK3的功能仍知之甚少。本研究结果表明：SlMAPK3参与了番茄对番茄黄化曲叶病毒的抗病毒反应，并且这个过程可能是通过水杨酸（SA）或者茉莉酸（JA）防御信号途径进行的。
外文摘要：Several recent studies have reported on the role of mitogen-activated protein kinase (MAPK3) in plant immune responses. However, little is known about how MAPK3 functions in tomato (Solanum lycopersicum L.) infected with tomato yellow leaf curl virus (TYLCV). There is also uncertainty about the connection between plant MAPK3 and the salicylic acid (SA) and jasmonic acid (JA) defense-signaling pathways. The results of this study indicated that SlMAPK3 participates in the antiviral response against TYLCV. Tomato seedlings were inoculated with TYLCV to investigate the possible roles of SlMAPK1, SlMAPK2, and SlMAPK3 against this virus. Inoculation with TYLCV strongly induced the expression and the activity of all three genes. Silencing of SlMAPK1, SlMAPK2, and SlMAPK3 reduced tolerance to TYLCV, increased leaf H2O2 concentrations, and attenuated expression of defense-related genes after TYLCV infection, especially in SlMAPK3-silenced plants. Exogenous SA and methyl jasmonic acid (MeJA) both significantly induced SlMAPK3 expression in tomato leaves. Over-expression of SlMAPK3 increased the transcript levels of SA/JA-mediated defense-related genes (PR1, PR1b/SlLapA, SlPI-I, and SlPI-II) and enhanced tolerance to TYLCV. After TYLCV inoculation, the leaves of SlMAPK3 over-expressed plants compared with wild type plants showed less H2O2 accumulation and greater superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) activity. Overall, the results suggested that SlMAPK3 participates in the antiviral response of tomato to TYLCV, and that this process may be through either the SA or JA defense-signaling pathways.
作者：Li, Yunzhou; Qin, Lei; Zhao, Jingjing; 等
作者单位：西北农林科技大学
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2017
出版刊次：2
点击下载：SlMAPK3通过介导水杨酸和茉莉酸信号增强番茄对番茄黄化曲叶病毒（TYLCV）的抗性