中文摘要：桃蚜是马铃薯卷叶病毒（PLRV）（仅由蚜虫以循环方式传播）的介体。萝卜饲养的桃蚜无性系血统与杂草酸浆饲养的相比，PLRV传播效率明显降低。本研究发现，半胱氨酸蛋白酶组织蛋白酶B （cathB）的化学抑制修复了萝卜饲养的蚜虫以剂量依赖的方式传播PLRV的能力，这表明cathB增加的能力和细胞膜间其他半胱氨酸蛋白酶间接地降低了蚜虫病毒传播。了解寄主植物如何影响蚜虫的病毒传播是种植者管理田间作物病毒传播的关键。
外文摘要：The green peach aphid, Myzus persicae, is a vector of the Potato leafroll virus (PLRV, Luteoviridae), transmitted exclusively by aphids in a circulative manner. PLRV transmission efficiency was significantly reduced when a clonal lineage of M. persicae was reared on turnip as compared to the weed physalis, a transient effect caused by a host-switch response. A trend of higher PLRV titer in physalis-reared aphids as compared to turnip-reared aphids was observed at 24h and 72h after virus acquisition. The major difference in the proteomes of these aphids was the upregulation of predicted lysosomal enzymes, in particular the cysteine protease cathepsin B (cathB), in aphids reared on turnip. The aphid midgut is the site of PLRV acquisition, and cathB and PLRV localization were starkly different in midguts of the aphids reared on the two host plants. In viruliferous aphids that were reared on turnip, there is near complete co-localization of cathB and PLRV at the cell membranes, which was not observed in physalis-reared aphids. Chemical inhibition of cathB restored the ability of aphids reared on turnip to transmit PLRV in a dose-dependent manner, showing that the increased activity of cathB and other cysteine proteases at the cell membrane indirectly decreases virus transmission by aphids. Understanding how the host plant influences virus transmission by aphids is critical for growers to manage the spread of virus among field crops.
作者：PV Pinheiro, M Ghanim, MMM Alexander, 等
作者单位：康奈尔大学
期刊名称：Molecular & Cellular Proteomics
期刊影响因子：5.912
出版年份：2016
出版刊次：12
点击下载：寄主植物通过改变蚜虫介体肠道半胱氨酸蛋白酶活性间接影响植物病毒的传播