中文摘要：新德里番茄曲叶病毒病（ToLCNDV）由烟粉虱自然传播，其导致的病害可对瓜类作物造成重大的经济损失。简单有效的接种方案和检测方法的可行性是筛选品种、种质资源收集和繁殖群体的必要条件。本文通过使用三种不同的缓冲液在易感西葫芦品种上进行机械汁液接种，评估了拟接种的ToLCNDV的侵染性。接种方法学和检测方法的有关信息与葫芦科作物耐受或抗ToLCNDV品种的筛选和育种有很大的相关性。
外文摘要：The disease caused by Tomato leaf curl New Delhi virus (ToLCNDV), which is naturally transmitted by the whitefly Bemisia tabaci, causes important economic losses in cucurbit crops. The availability of simple and efficient inoculation protocols and detection methods is necessary for screening varieties and germplasm collections as well as for breeding populations. We evaluated the infectivity of ToLCNDV inocula prepared using three different buffers for mechanical sap inoculation in a susceptible variety of zucchini. We found that inoculum prepared with buffer III, which contains polyvinylpyrrolidone, is highly efficient for mechanical inoculation, with 100% of plants displaying severe symptoms 21 days post-inoculation. Using this buffer, we mechanically inoculated 19 commercial varieties of cucurbit crops (six of cucumber, six of melon and seven of zucchini), evaluated the evolution of symptoms and diagnosed infection using nine different ToLCNDV detection methods (four based on serology, four based on molecular hybridization and one based on PCR detection). The results revealed that all varieties are susceptible, although cucumber varieties display less severe symptoms than those of melon or zucchini. All detection methods were highly efficient (more than 85% of plants testing positive) in melon and zucchini, but in cucumber, the percentage of positive plants detected with serology and molecular hybridization methods ranged from 20.4% with Squash leaf curl virus (SLCV) antiserum, to 78.5% with DNA extract hybridization. Overall, the best detection results were obtained with PCR, with 92.6%, 92.4% and 98.4% cucumber, melon and zucchini plants, respectively, testing positive. When considering the overall results in the three crops, the best serology and molecular hybridization methods were those using Watermelon chlorotic stunt virus (WmCSV) antiserum and DNA extract, respectively. The inoculation methodology developed and the information on detection methods are of great relevance for the selection and breeding of varieties of cucurbit crops that are tolerant or resistant to ToLCNDV.
外文关键词：Cucurbits; molecular hybridization; PCR; sap inoculation; serology; ToLCNDV.
作者：MR Figàs, A Alfaro‐Fernández, MI Font, 等
作者单位：瓦伦西亚理工大学
期刊名称：Annals of Applied Biology
期刊影响因子：2.103
出版年份：2017
出版刊次：3
点击下载：在黄瓜、甜瓜和西葫芦品种上接种新德里番茄曲叶病毒并用不同的检测方法评估病毒侵染的情况研究