中文摘要：从2014年和2015年南韩全国范围内辣椒地调查中筛选出两个辣椒轻斑驳病毒(PMMoV)染病隔离种群，这两个PMMoV隔离种群（Sangcheong 47 (S-47, KX399390)和Jeongsong 76 (J-76, KX399389)）共享99%的核苷酸和氨基酸，并且在核苷酸水平上与日本种和中国种很接近。隔离种群间126/183 kDa相关功能（包括亚细胞定位）的差异表明，与寄主蛋白质间不同的交互作用可能会影响辣椒症状的发展。
外文摘要：Two isolates of Pepper mild mottle virus (PMMoV) were selected from a nationwide survey of pepper fields in South Korea in 2014 and 2015, in which Cucumber mosaic virus was also detected; the two PMMoV isolates, Sangcheong 47 (S-47, KX399390) and Jeongsong 76 (J-76, KX399389), share ~99% nucleotide and amino acid identity and are closely related to Japanese and Chinese isolates at the nucleotide level. Amino acid sequence comparisons revealed 99.73, 99.81, 98.44, and 100% identity in the ORF1, ORF2, MP, and CP, respectively, between S-47 and J-76. In addition, we generated infectious clones of S-47 and J-76, and T7 promoter driven transcripts of each inoculated to Nicotiana benthamiana produced very severe symptoms, whereas only mild symptoms developed in Capsicum annuum. Gene silencing suppressor function of 126 kDa and cytoskeleton-connected plasmodesmata localization of movement protein of S-47 and J-76 showed no difference between isolates, whereas 126 kDa of J-76 clearly formed intracellular aggregates not observed with S-47 126 kDa protein. Differences between these isolates in 126/183 kDa-related functions including subcellular localization suggest that differential interactions with host proteins may affect symptom development in C. annuum.
外文关键词：Nationwide survey, Full-length infectious cDNA clone, T7 promoter, In vitro transcription, Seed transmission
作者：SH Han, JS Park, JY Han, 等
作者单位：韩国忠南国立大学
期刊名称：Virus Genes
期刊影响因子：1.285
出版年份：2017
出版刊次：2
点击下载：韩国辣椒轻斑驳病毒（PMMoV）隔离种群（在症状严重程度及126 kDa蛋白亚细胞定位方面有所不同）研究