中文摘要：蚜虫（包括多面手植食性昆虫桃蚜）可传播黄瓜花叶病毒（CMV）。CMV（Fny菌株）侵染可以不同的方式影响桃蚜在烟草、拟南芥和葫芦上的取食行为和表现。在拟南芥和葫芦上，CMV可降低寄主质量并可抑制长期饲养的蚜虫（这些蚜虫可增强病毒传播速率）。被CMV感染的葫芦也会释放出具有欺骗性的吸引蚜虫的挥发物（有利于病毒的采集）。本研究结果表明，烟草植株感染CMV引起寄主排放的挥发性物质数量和质量的变化，这些改变部分取决于2b抗性蛋白的活性。虽然CMV侵染的植株上桃蚜寄主质量较高，但CMV引起的烟草植株挥发物排放的改变并没有明显影响蚜虫在感染和模拟接种植株上的沉降。
外文摘要：Background: Aphids, including the generalist herbivore Myzus persicae, transmit cucumber mosaic virus (CMV). CMV (strain Fny) infection affects M. persicae feeding behavior and performance on tobacco (Nicotiana tabacum), Arabidopsis thaliana and cucurbits in varying ways. In Arabidopsis and cucurbits, CMV decreases host quality and inhibits prolonged feeding by aphids, which may enhance virus transmission rates. CMV-infected cucurbits also emit deceptive, aphid-attracting volatiles, which may favor virus acquisition. In contrast, aphids on CMV-infected tobacco (cv. Xanthi) exhibit increased survival and reproduction. This may not increase transmission but might increase virus and vector persistence within plant communities. The CMV 2b counter-defense protein diminishes resistance to aphid infestation in CMV-infected tobacco plants. We hypothesised that in tobacco CMV and its 2b protein might also alter the emission of volatile organic compounds that would influence aphid behavior. Results: Analysis of headspace volatiles emitted from tobacco plants showed that CMV infection both increased the total quantity and altered the blend produced. Furthermore, experiments with a CMV 2b gene deletion mutant (CMVΔ2b) showed that the 2b counter-defense protein influences volatile emission. Free choice bioassays were conducted where wingless M. persicae could choose to settle on infected or mock-inoculated plants under a normal day/night regime or in continual darkness. Settling was recorded at 15 min, 1 h and 24 h post-release. Statistical analysis indicated that aphids showed no marked preference to settle on mock-inoculated versus infected plants, except for a marginally greater settlement of aphids on mock-inoculated over CMV-infected plants under normal illumination. Conclusions: CMV infection of tobacco plants induced quantitative and qualitative changes in host volatile emission and these changes depended in part on the activity of the 2b counter-defense protein. However, CMV-induced alterations in tobacco plant volatile emission did not have marked effects on the settling of aphids on infected versus mock-inoculated plants even though CMV-infected plants are higher quality hosts for M. persicae.
外文关键词：RNA silencing, 2b protein, Systemic induced resistance, Induced susceptibility, Plant-herbivore interactions, Plant-virus interactions, Secondary metabolism, Semiochemical, Jasmonates, Cucumovirus, Potyvirus
作者：T Tungadi, SC Groen, AM Murphy, 等
作者单位：剑桥大学
期刊名称：Virology Journal
期刊影响因子：2.362
出版年份：2017
出版刊次：5
点击下载：黄瓜花叶病毒及其2b蛋白改变了寄主体内挥发性有机化合物的排放但没有改变蚜虫介体在烟草上的沉降