中文摘要：鞘脂类及其代谢物与哺乳动物细胞内病毒侵染和复制有关，但是寄主体内代谢酶如何受病毒调节仍然还有很多未知。本研究报道了12种鞘脂类基因的鉴定以及灰飞虱（整个东亚水稻上较严重的害虫）体内水稻条纹病毒对它们的调节。根据蛋白质序列相似性，作者鉴定了灰飞虱体内12种鞘脂酶基因。通过采用qPCR比较不同生命阶段带毒与非带毒灰飞虱体内mRNA水平，发现在灰飞虱大多数阶段RSV侵染上调6个基因（LsCGT1, LsNAGA1, LsSGPP, LsSMPD4, LsSMS和LsSPT）。特别是在带毒灰飞虱3龄和4龄若虫阶段，分别有4个（LsCGT1, LsSMPD2, LsNAGA1, and LsSMS）和另3个（LsNAGA1, LsSGPP, and LsSMS）基因明显被下调。HPLC-MS/MS结果表明，RSV侵染增加了灰飞虱3龄和4龄若虫各种神经酰胺的水平，如Cer18:0, Cer20:0和Cer22:0等。总的来说，这些研究结果表明，RSV侵染改变了各种鞘脂酶的转录水平和灰飞虱体内鞘脂含量，这意味着鞘脂可能对RSV侵染或在灰飞虱体内的复制很重要。
外文摘要：Sphingolipids and their metabolites have been implicated in viral infection and replication in mammal cells but how their metabolizing enzymes in the host are regulated by viruses remains largely unknown. Here we report the identification of 12 sphingolipid genes and their regulation by Rice stripe virus in the small brown planthopper (Laodelphax striatellus Fallen), a serious pest of rice throughout eastern Asia. According to protein sequence similarity, we identified 12 sphingolipid enzyme genes in L. striatellus. By comparing their mRNA levels in viruliferous versus nonviruliferous L. striatellus at different life stages by qPCR, we found that RSV infection upregulated six genes ( LsCGT1, LsNAGA1, LsSGPP, LsSMPD4, LsSMS, and LsSPT) in most stages of L. striatellus. Especially, four genes ( LsCGT1, LsSMPD2, LsNAGA1, and LsSMS) and another three genes ( LsNAGA1, LsSGPP, and LsSMS) were significantly upregulated in viruliferous third-instar and fourth-instar nymphs, respectively. HPLC-MS/MS results showed that RSV infection increased the levels of various ceramides, such as Cer18:0, Cer20:0, and Cer22:0 species, in third and fourth instar L. striatellus nymphs. Together, these results demonstrate that RSV infection alters the transcript levels of various sphingolipid enzymes and the contents of sphingolipids in L. striatellus, indicating that sphingolipids may be important for RSV infection or replication in L. striatellus.
外文关键词：ceramide; sphingomyelin synthase; rice stripe virus; serine palmitoyltransferase; HPLC-MS/MS
作者：Jiao, Wen-Juan; Li, Fei-Qiang; Bai, Yue-Liang; 等
作者单位：浙江大学
期刊名称：JOURNAL OF INSECT SCIENCE
期刊影响因子：0.843
出版年份：2017
出版刊次：1
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