中文摘要：水稻条纹病毒（RSV）是一种丝状、负链RNA病毒，可在亚洲国家水稻上引起严重的病害。病毒颗粒主要由核衣壳蛋白（NP）和基因组RNA组成。然而，在病毒颗粒组装过程中RSV NP如何与基因组RNA交互作用的分子细节仍很不清楚。本研究为 RSV NPs与基因组RNA交互作用分子机制提供了有价值的新见解。
外文摘要：Rice stripe tenuivirus (RSV) is a filamentous, negative-strand RNA virus causing severe diseases on rice in Asian countries. The viral particle is composed predominantly of a nucleocapsid protein (NP) and genomic RNA. However, the molecular details of how the RSV NP interacts with genomic RNA during particle assembly remain largely unknown. Here, we modeled the NP-RNA complex and show that polar amino acids within a predicted groove of NP are critical for RNA binding and protecting the RNA from RNase digestion. RSV NP formed pentamers, hexamers, heptamers, and octamers. By modeling the higher-order structures, we found that oligomer formation was driven by the N-terminal amino arm of the NP. Deletion of this arm abolished oligomerization; the N-terminally truncated NP was less able to interact with RNA and protect RNA than was the wild type. These findings afford valuable new insights into molecular mechanism of RSV NPs interacting with genomic RNA.
外文关键词：Model-based structure-function analysis; Rice Stripe Tenuivirus; Nucleocapsid Protein; RNA-binding sites
作者：Lu, Gang; Li, Jia; Zhou, Yijun; 等
作者单位：南京农业大学
期刊名称：VIROLOGY
期刊影响因子：3.353
出版年份：2017
出版刊次：6
点击下载：基于模型的与病毒基因组RNA交互作用的水稻条纹病毒核衣壳蛋白结构与功能特征研究