番茄褪绿病毒经小叶嫁接进行有效传播的研究

Efficient Transmission and Propagation of Tomato Chlorosis Virus by Simple Single-Leaflet Grafting

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中文摘要:番茄褪绿病毒(ToCV)是毛形病毒属成员之一,可引起全球番茄出现流行病害。ToCV仅局限于韧皮部,由烟粉虱以半持久方式传播,但不通过机械接种传播。ToCV的试验性传播主要是通过利用粉虱作为媒介进行的接种传播。为了开发一种简单、便捷的ToCV传播方法,本文将感染ToCV的番茄植株单小叶嫁接至番茄幼苗上进行研究。研究结果表明,单小叶嫁接可以提供足够数量的病毒接种源让ToCV成功传播到嫁接的幼苗上。
外文摘要:Tomato chlorosis virus (ToCV), a member of the genus Crinivirus, has caused an epidemic disease in tomato worldwide. ToCV is phloem-limited and transmitted by whiteflies in a semi-persistent manner, but not by mechanical inoculation. Experimental propagation of ToCV has been performed primarily by using whitefly-mediated inoculation. To develop a simple and convenient method for transmission of ToCV, we investigated grafting single-leaflets from tomato plants infected with ToCV to recipient tomato seedlings. Forty-one of 46 tomato seedlings tested were grafted successfully with single-leaflets infected with ToCV. Among them, 36 seedlings (87.8%) were systemically infected with ToCV and developed typical symptoms. Our results demonstrated that single-leaflet grafting could provide a sufficient amount of inoculum for the transmission of ToCV to the grafted seedlings.
外文关键词:graft inoculation, single-leaflet, tomato chlorosis virus
作者:Lee, Huin; Kim, Mi-Kyeong; Choi, Hong-Soo; 等
作者单位:南朝鲜全北国立大学
期刊名称:Plant Pathology Journal
期刊影响因子:1.255
出版年份:2017
出版刊次:6
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  1. 编译服务:植物病毒学
  2. 编译者:戴红君
  3. 编译时间:2017-07-13