中文摘要：2014-2015年，在德克萨斯南部一个20公顷果园的木瓜树上发现了严重的类病毒症状，主要包含了斑驳、扭曲、黄化和变脆等。发病6个月内有症状的植株发病率从40%上升到100%；受影响最严重的植株显得很矮小，果实产量与质量比无症状的植株少。果园木瓜植株上的病毒组是通过采用Illumina NextSeq 500平台进行检测的，检测的结果通过对PCR扩增得到的整个病毒基因组进行Sanger DNA测序来验证。结果显示，木瓜上存在着木瓜环斑病毒（PRSV；马铃薯Y病毒组）、莴苣黄化病毒（LCV；长线形病毒属）和番茄黄化曲叶病毒- IL（TYLCV-IL；菜豆金黄花叶病毒属）。木瓜是多年生植物，充当起了TYLCV-IL和LCV的储存库，这使得木瓜园里病毒病害的管理面临着新挑战。
外文摘要：Severe virus-like symptoms consisting of mosaic, distortion, yellowing, and brittleness were observed on papaya plants in a 20-ha orchard in South Texas during the 2014-15 growing season. Incidence of symptomatic plants increased from 40 to 100% within 6 months of the outbreak; the most severely affected plants were stunted, and fruit yield and quality were reduced compared with asymptomatic plants. The orchard papaya plant virome was explored using the Illumina NextSeq 500 platform and results were validated by Sanger DNA sequencing of complete viral genomes obtained by PCR amplification. The combined results revealed the presence of Papaya ringspot virus (PRSV; Potyvirus), Lettuce chlorosis virus (LCV; Crinivirus), and Tomato yellow leaf curl virus-IL (TYLCV-IL; Begomovirus). The RT-PCR analyses of leaves from 51 randomly sampled papaya plants indicated the presence of PRSV, LCV, and TYLCV-IL in 100, 39.2, and 15.7% of the samples, respectively. Plants infected with PRSV, in combination with LCV and/or TYLCV-1L, exhibited more severe symptoms compared with plants infected with PRSV alone. Furthermore, successful whitefly-mediated transmission of TYLCV-IL and LCV was accomplished by exposing virus-free papaya seedlings to viruliferous Bernisia tabaci (Genn.) under greenhouse conditions. The results of this study document a new host record for LCV and the first successful whitefly-mediated transmission of TYLCV-IL and LCV to papaya. As a perennial crop, infected papaya serving as an over-seasoning reservoir for TYLCV-IL and LCV, presents a new challenge to viral disease management in papaya orchards.
外文关键词：Whitefly, papaya, epidemiology, mixed virus infections, alternative host Crinivirus, Potyvirus, Papaya ringspot virus, Begomovirus, Geminiviridae
作者：Alabi, Olufemi J.; Al Rwahnih, M.; Jifon, J. L.; 等
作者单位：德克萨斯A&M的农业研究和推广中心
期刊名称：PLANT DISEASE
期刊影响因子：0.0
出版年份：2017
出版刊次：7
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