中文摘要：植物激素在植物免疫反应中起着重要的作用。然而，与双子叶植物激素调节免疫力方面的大量信息相比较，单子叶植物病毒防御系统方面可用的信息非常少。本文利用高通量测序法比较了感染水稻黑条矮缩病毒（RBSDV）水稻与健康水稻所有基因的表达。利用外源激素和转基因水稻来检测RBSDV病毒的传染性和致病性。研究结果显示，感染病毒植株中茉莉酸（JA）途径被诱导，而油菜素内酯（BR）途径被抑制。总的来说，研究结果表明，JA介导的防御可以抑制BR介导的对RBSDV侵染的敏感性。
外文摘要：Plant hormones play a vital role in plant immune responses. However, in contrast to the relative wealth of information on hormone-mediated immunity in dicot plants, little information is available on monocot-virus defense systems. We used a high-throughput-sequencing approach to compare the global gene expression of Rice black-streaked dwarf virus (RBSDV)-infected rice plants with that of healthy plants. Exogenous hormone applications and transgenic rice were used to test RBSDV infectivity and pathogenicity. Our results revealed that the jasmonic acid (JA) pathway was induced while the brassinosteroid (BR) pathway was suppressed in infected plants. Foliar application of methyl jasmonate (MeJA) or brassinazole (BRZ) resulted in a significant reduction in RBSDV incidence, while epibrassinolide (BL) treatment increased RBSDV infection. Infection studies using coi1-13 and Go mutants demonstrated JA-mediated resistance and BR-mediated susceptibility to RBSDV infection. A mixture of MeJA and BL treatment resulted in a significant reduction in RBSDV infection compared with a single BL treatment. MeJA application efficiently suppressed the expression of BR pathway genes, and this inhibition depended on the JA coreceptor OsCOI1. Collectively, our results reveal that JA-mediated defense can suppress the BR-mediated susceptibility to RBSDV infection.
外文关键词：brassinosteroid pathway; jasmonic acid pathway; Oryza sativa (rice); plant defense response; Rice Black Streaked Dwarf Virus (RBSDV)
作者：He, Yuqing; Zhang, Hehong; Sun, Zongtao; 等
作者单位：浙江农业科学院
期刊名称：NEW PHYTOLOGIST
期刊影响因子：6.72
出版年份：2017
出版刊次：4
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