中文摘要：大多数植物病毒由昆虫介体在植物间传播，病毒从介体亲本经卵巢向后代传播对病毒的流行病学有着重要的意义。菜豆金黄花叶病毒属病毒由烟粉虱以循环的方式传播，并通过植物-昆虫-植物循环的方式维持。对于菜豆金黄花叶病毒属病毒其他的传播路径还不太清楚。本文研究发现，番茄黄化曲叶病毒（TYLCV）经常会经烟粉虱雌虫卵巢向后代传播，且这种传播方式明显有助于病毒的全球传播。研究结果表明，在缺少染毒植株的情况下TYLCV至少可以持续两代，并且在实验室和大田中成虫后代都可能会侵染健康植株。本文报道了菜豆金黄花叶病毒属病毒经卵巢传播机制，或许有助于解释某些菜豆金黄花叶病毒属病毒的演变和全球传播。
外文摘要：The majority of plant viruses are transmitted by insect vectors between hosts, and transovarial transmission of viruses from vector parents to offspring has great significance to their epidemiology. Begomoviruses are transmitted by the whitefly Bemisia tabaci in a circulative manner and are maintained through a plant-insect-plant cycle. Other routes of begomovirus transmission are not clearly known. Here, we report that transovarial transmission from female whiteflies to offspring often happens for one begomovirus, Tomato yellow leaf curl virus (TYLCV), and may have contributed significantly to its global spread. We found that TYLCV entry of the reproductive organ of its vector mainly depended on the developmental stage of the whitefly ovary, and the transovarial transmission of TYLCV to offspring increased with whitefly adult age. The specific interaction between virus coat protein (CP) and whitefly vitellogenin (Vg) was vital for virus entry into whitefly ovary. When knocking down the expression of Vg, the entry of TYLCV into ovary was inhibited and the transovarial transmission efficiency decreased. In contrast, another begomovirus, Papaya leaf curl China virus (PaLCuCNV), CP did not interact with whitefly Vg, and PaLCuCNV could not be transovarially transmitted by whiteflies. We further showed that TYLCV could be maintained for at least two generations in the absence of virusinfected plants, and the adult progenies were able to infect healthy plants in both the laboratory and field. This study reports the transovarial transmission mechanism of begomoviruses, and it may help to explain the evolution and global spread of some begomoviruses.
外文关键词：begomovirus, transovarial transmission, vector development, vitellogenin, whitefly
作者：Wei, Jing; He, Ya-Zhou; Guo, Qi; 等
作者单位：浙江大学
期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
期刊影响因子：8.56
出版年份：2017
出版刊次：6
点击下载：介体的发育和卵黄蛋白原决定了菜豆金黄花叶病毒属病毒经卵巢传播的方式