中文摘要：过去的几年中，南方水稻黑条矮缩病毒（SRBSDV）已经从中国南方传播到越南北部，严重影响了水稻生产。SRBSDV潜伏期较长，早期症状不明显，因此，控制该病毒比较困难。壳寡糖（COS）是一种绿色的植物免疫调节剂。早期的研究表明，预防和控制SRBSDV有一定的效果，并且可降低病害感染率，但其潜在的控制和预防机制尚不清楚。本文利用无标记蛋白质组学方法分析COS处理后水稻差异表达的蛋白质。结果表明，COS可以上调植物防御相关蛋白、下调SRBSDV蛋白的表达水平。同时，定量实时PCR检测结果表明，COS可以提高水稻防御基因的表达。此外，COS可通过丝裂原活化蛋白激酶信号级联途径增强过氧化物酶、超氧化物歧化酶和过氧化氢酶的防御酶活性，增强水稻抗病性。
外文摘要：Southern rice black-streaked dwarf virus (SRBSDV) has spread from the south of China to the north of Vietnam in the past few years and severely influenced rice production. Its long incubation period and early symptoms are not evident; thus, controlling it is difficult. Chitosan oligosaccharide (COS) is a green plant immunomodulator. Early studies showed that preventing and controlling SRBSDV have a certain effect and reduce disease infection rate, but its underlying controlling and preventing mechanism is unclear. In this study, label-free proteomics was used to analyze differentially expressed proteins in rice after COS treatment. The results showed that COS can up-regulate the plant defense-related proteins and down-regulate the protein expression levels of SRBSDV. Meanwhile, quantitative real-time PCR test results showed that COS can improve defense gene expression in rice. Moreover, COS can enhance the defense enzymatic activities of peroxidase, superoxide dismutase and catalase through mitogen-activated protein kinase signaling cascade pathway, and enhance the rice disease resistance.
外文关键词：chitosan oligosaccharide, Southern rice black-streaked dwarf virus, label-free proteomics, mechanism, defensive reaction, catalase
作者：Yang, Anming; Yu, Lu; Chen, Zhuo; 等
作者单位：贵州大学
期刊名称：VIRUSES-BASEL
期刊影响因子：3.6
出版年份：2017
出版刊次：5
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