中文摘要：烟粉虱被认为是农业上最重要的害虫之一，与之相关的一个重要问题就是烟粉虱是植物病毒尤其是菜豆金色花叶病毒属病毒的介体。作者之前已鉴定了烟粉虱MEAM1体内的类防御素抗菌肽Btdef。但是，Btdef在昆虫介体免疫系统和菜豆金色花叶病毒属病毒传播中的功能还有待探索。本研究发现，Btdef可能通过菜豆金色花叶病毒属病毒与烟粉虱之间互作共生调节的改变参与了菜豆金色花叶病毒属病毒的侵染。因此，可以Btdef为目标，将其作为控制烟粉虱传播的菜豆金色花叶病毒属病毒的新策略。
外文摘要：BACKGROUND: The whitefly Bemisia tabaci (Gennadius) is considered one of the main pests for agriculture. One important problem with the whitefly is its notorious status as a vector for plant viruses, primarily begomoviruses. We have previously identified a defensin-like antimicrobial peptide, Btdef, from the whitefly B. tabaci MEAM1. However, the function of Btdef in the immune system of the insect vector and begomovirus transmission has yet to be explored. RESULTS: To explore the role of Btdef during begomovirus transmission, we firstly investigated the transcriptional response of Btdef following acquisition of Tomato yellow leaf curl China virus (TYLCCNV). The expression of Btdef was up-regulated in the viruliferous whiteflies. After RNA silencing of the Btdef gene in adult whiteflies fed with dsRNA, they were allowed to feed on TYLCCNV-infected plants and then quantified for TYLCCNV DNA titre. Unexpectedly, silencing the Btdef gene reduced both the abundance and expressions of TYLCCNV genes in the whiteflies. In the meantime, the density of the endosymbiont Rickettsia was significantly reduced in dsBtdef-fed whiteflies. CONCLUSION: Our data provide evidence that Btdef is involved in begomovirus infection, possibly through symbiont-mediated alteration of begomovirus-whitefly interactions. These findings indicate that Btdef may be targeted for the development of new technology for the control of whitefly-transmitted begomoviruses.
外文关键词：whitefly; defensin; Begomovirus; TYLCCNV; symbionts
作者：Wang, Zhi-zhi; Bing, Xiao-li; Liu, Shu-sheng; 等
作者单位：浙江大学
期刊名称：PEST MANAGEMENT SCIENCE
期刊影响因子：2.96
出版年份：2017
出版刊次：7
点击下载：抗菌肽Btdef RNA干扰减少了中国番茄黄化曲叶病毒在烟粉虱体内的积累