中文摘要：灰飞虱（SBPH）是一种重要的病毒载体，可传播水稻条纹病毒（RSV）。昆虫共生体对昆虫适应性起着至关重要的作用，然而，目前昆虫共生体对SBPH病毒传播的作用仍然未知。本文利用Illumina 16S rRNA基因MiSeq测序技术研究了非带毒、感染RSV、感染RBSDV的SBPH种群中的内共生菌群落。序列分析结果揭示了不同SBPH种群中不同分类水平的菌群百分比。细菌多样性和丰富度极其一致，表明RSV或RBSDV侵染并没有影响SBPH共生菌的组成与丰富度。值得一提的是，沃尔巴克氏体在所有种群中占绝对优势。将来可通过研究沃尔巴克氏体与SBPH之间的共生关系来控制害虫SBPH。
外文摘要：The small brown planthopper (SBPH) is an important virus vector, transmitting Rice stripe virus (RSV), and Rice black-streaked dwarf virus (RBSDV). Insect symbionts play an essential role in the insect fitness, however, it is still unclear about their contributions to viral transmission by SBPH. Here, we investigated endosymbiont communities in non-viruliferous, RSV-infected, and RBSDV-infected SBPH populations using Illumina 16S rRNA gene MiSeq sequencing. In total, 281,803 effective sequences of the 16S rRNA gene were generated from different samples. Sequence analysis revealed the percentages of these bacterial groups in different SBPH populations on several taxonomic levels ranging from phyla to genera. The extremely consistent bacterial diversity and abundance indicated that RSV or RBSDV infection did not affect the composition and abundance of symbionts in SBPH. It was notable that Wolbachia was dominant in all populations. The symbiosis between Wolbachia and SBPH might be potentially studied and utilized to control pest SBPH in the future.
外文关键词：endosymbiont community; rice black-streaked dwarf virus; rice stripe virus; small brown planthopper
作者：Li, Shuo; Zhou, Changwei; Chen, Guangyi; 等
作者单位：江苏省农业科学院
期刊名称：JOURNAL OF BASIC MICROBIOLOGY
期刊影响因子：1.61
出版年份：2017
出版刊次：7
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