中文摘要：本文研究描述了一种新的假定的豌豆耳突花叶病毒组成员，即葡萄豌豆耳突花叶病毒-1（GEV-1），该病毒是通过高通量测序（HTS）发现的。利用HTS对来自巴西南部、东南部和东北部地区的17种葡萄树进行了调查，发现只有在南部地区葡萄树上有GEV-1，该病毒在南部主要侵染4种葡萄树（赤霞珠，赛美蓉，CG 90450和品丽珠），且核苷酸水平都非常一致地保持在99%左右。这种新型病毒只有在多病毒侵染表现出病毒样症状的植株上才能检测到。这是首例黄症病毒科病毒侵染葡萄树的报道。基于基因组学特性和系统发育分析，GEV-1应该被归类为豌豆耳突花叶病毒组属一新成员。
外文摘要：In this study, we describe a novel putative Enamovirus member, Grapevine enamovirus-1 (GEV-1), discovered by high-throughput sequencing (HTS). A limited survey using HTS of 17 grapevines (Vitis spp.) from the south, southeast, and northeast regions of Brazil led to the detection of GEV-1 exclusively on southern plants, infecting four grapevine cultivars (Cabernet Sauvignon, Semillon, CG 90450, and Cabernet franc) with a remarkable identity of around 99% at the nucleotide level. This novel virus was only detected in multiple-virus infected plants exhibiting viral-like symptoms. GEV-1 was also detected on a cv. Malvasia Longa by RT-PCR. We performed graft-transmissibility assays on GEV-1. The organization, products, and cis-acting regulatory elements of GEV-1 genome are also discussed here. The near complete genome sequence of GEV-1 was obtained during the course of this study, lacking only part of the 3' untranslated terminal region. This is the first report of a virus in the family Luteoviridae infecting grapevines. Based on its genomic properties and phylogenetic analyses, GEV-1 should be classified as a new member of the genus Enamovirus.
外文关键词：High-throughput sequencing; Virus discovery; Luteoviridae; Grapevine enamovirus-1; GEV-1
作者：Fagundes Silva, Joao Marcos; Al Rwahnih, Maher; Blawid, Rosana; 等
作者单位：Embrapa Grape & Wine, Bento Goncalves, RS, Brazil
期刊名称：VIRUS GENES
期刊影响因子：1.55
出版年份：2017
出版刊次：8
点击下载：一种新的豌豆耳突花叶病毒组病毒即葡萄豌豆耳突花叶病毒-1的发现与分子特征研究