中文摘要：广泛使用的抗性辣椒触发了番茄斑萎病毒（TSWV）抗性-破坏（RB）分离株在全球范围内快速出现。虽然TSWV诱导的病毒已经在云南快速增长，但近几年没有关于该区域TSWV多样性的可用信息。本文研究报道了云南收集所得的分离株中TSWV RB自然突变体的出现。不同RB分离株的出现以及Tsw介导的抗性控制的失败对中国西南部地区辣椒构成了严重威胁。
外文摘要：Widely used resistant peppers (Capsicum spp.) bearing the Tsw locus triggered the rapid emergence of resistance-breaking (RB) isolates of Tomato spotted wilt virus (TSWV) around the world. However, although TSWV-induced diseases have rapidly increased in Yunnan, southwest China, in recent years, no information is available about the diversity of TSWV isolates in this region. In this study, the occurrence of natural TSWV RB variants among isolates collected in Yunnan is reported. Initially, a TSWV isolate from asparagus lettuce (TSWV-LE) was collected in Yunnan in 2012. Surprisingly, this isolate of TSWV induced systemic necrosis on pepper carrying the Tsw resistance gene. Novel TSWV isolates, collected in 2015, included a tomato isolate (TSWV-YN18) and a tobacco isolate (TSWV-YN53) that also overcame Tsw-mediated resistance. TSWV-YN18 induced systemic ringspots, whereas TSWV-YN53 caused systemic chlorotic mottling. Variations in the TSWV nonstructural (NSs) protein are the key determinants associated with Tsw resistance-breaking isolates. It was found that TSWV-LE NSs retained the hypersensitive response (HR) induction, whereas TSWV-YN18 and TSWV-YN53 NSs were unable to induce HR. However, the NSs of all three RB isolates suppressed RNA silencing. Sequence analysis of the NSs revealed that RB isolates of Yunnan have no amino acid mutation sites common to other previously reported RB isolates. However, two amino acids (F74 and K272) on TSWV-LE NSs make it distinct from TSWV-YN18 and TSWV-YN53. The occurrence of different RB isolates and the failure of Tsw-mediated resistance control pose serious threats to domestic pepper crops in southwest China.
外文关键词：Capsicum; diversity; resistance-breaking isolates; suppression of RNA silencing; Tomato spotted wilt virus; Tsw resistance gene
作者：Jiang, L.; Huang, Y.; Sun, L.; 等
作者单位：南京农业大学
期刊名称：PLANT PATHOLOGY
期刊影响因子：2.46
出版年份：2017
出版刊次：8
点击下载：云南破坏辣椒番茄斑萎病（Tsw）抗性基因的番茄斑萎病毒株的出现与多样性研究