中文摘要：本研究目的在于确定烟草不同AGO蛋白（NbAGOs）对防御基于番茄丛矮病毒（TBSV）（番茄丛矮病毒组）、柽麻花叶病毒（烟草花叶病毒组）和狼尾草花叶病毒（马铃薯x病毒组）的敏感GFP表达病毒性构建沉默的贡献。根据烟草脆裂病毒（TRV）介导的NbAGOJ、4、5或6的下调可见，对任何病毒都没有影响，然而NbAGO2的降低抑制了TGdP19的沉默。一种被称为NbAGO5L的新基因的下调显示出TGdP19沉默有所减少，但对其他两种病毒没有影响，然而NbAGO7的沉默导致了对三种病毒敏感性均有微量提高。总之，本研究揭示了新的抗病毒物质NbAGOs以及NbAGOJ沉默的显性效应。
外文摘要：The objective of this study was to determine the contribution of different ARGONAUTE proteins in Nicotiana benthamiana (NbAGOs) to the defense against silencing sensitive GFP-expressing viral constructs based on Tomato bushy stunt virus (TBSV) (Tombusvirus), Sunn-hemp mosaic virus (Tobamovirus), and Foxtail mosaic virus (Potexvirus). Upon Tobacco rattle virus (TRV)-mediated down-regulation of NbAGOJ, 4, 5, or 6, no effects were noted on susceptibility to any virus construct, whereas knockdown of NbAGO2 specifically prevented silencing of P19 defective TBSV (TGdP19). Down-regulation of a new gene referred to as NbAGO5L showed some reduced silencing for TGdP19 but not for the other two virus constructs, whereas silencing of NbAGO7 gave rise to a subtle increase in susceptibility to all three viruses. Co-infiltrating different TRV-NbAGO constructs simultaneously did not enhance virus susceptibility. However, an unexpected finding was that whenever the TRV-NbAGO1 construct was present, this compromised silencing of genes targeted by co-infiltrated constructs, as shown upon co-infiltration of TRV-NbAGOJ with either TRV-NbAGO2 or TRV-Sul (targeting Magnesium chelatase I). Only after a prolonged period (approximately 2 months) did TRV-Su/mediated systemic bleaching occur in these co-infected plants, suggesting that TRV-NbAGOJ hinders the silencing ability of other TRV-NbAGO constructs. In conclusion, this study revealed new antiviral NbAGOs and dominant effects of silencing NbAGOJ.
作者：Odokonyero, Denis；Mendoza, Maria R.；Moffett, Peter；等
作者单位：德克萨斯A&M大学
期刊名称：PHYTOPATHOLOGY
期刊影响因子：2.896
出版年份：2017
出版刊次：8
点击下载：烟草脆裂病毒（TRV）介导的本氏烟草AGO蛋白家族（NbAGOs）沉默揭示了新的抗病毒候选物质和TRV-NbAGO1的显性效应