中文摘要：病毒感染在全球范围内诱导和抑制了宿主基因表达。水稻条纹病毒（RSV）是纤细病毒属模式种，可侵染水稻和拟南芥植物。基于基因芯片和下一代测序的转录组学方法已被应用于研究水稻和RSV的交互作用。然而，目前对于拟南芥植物对RSV侵染反应方面的认知有限，因此需要进一步研究确定感染RSV的单子叶植物和双子叶植物之间病毒-宿主交互作用的相似或不同之处。本文利用基于数字基因表达（DGE）的RNA测序分析研究了拟南芥感染RSV后转录组的变化特征，在感染14天和21天后对感染RSV的样本转录组物与模拟处理样本进行比较。结果在感染RSV14天和21天后的拟南芥中发现了624个差异表达基因（DEGs），其中有255个转录被诱导，38个转录被抑制。研究结果表明，与宿主防御系统相关基因的转录在病症发展早期（感染RSV14天后）就已经被激活了，然而在感染期（感染RSV21天后）宿主防御反应系统被抑制。本研究中鉴定的DEGs，尤其是防御反应基因，适用于RSV-拟南芥交互作用的进一步功能分析研究。
外文摘要：Background: Virus infection induces and suppresses host gene expression on a global level. Rice stripe virus (RSV) is the type species of the genus Tenuivirus and infects rice and Arabidopsis plants. Microarray-based and next generation sequencing-based transcriptomic approaches have been used to study rice-RSV interactions. However, our knowledge of the response of Arabidopsis plants to RSV infection is limited, and it requires further investigation to determine the similarities (or differences) in virus-host interactions between monocot and dicot hosts infected with RSV. Methods: We characterized transcriptome changes in Arabidopsis thaliana infected with rice stripe virus (RSV) with RNAseq based digital gene expression (DGE) analysis. The transcriptomes of RSV-infected samples were compared to those of mock-treated samples at 14 and 21 days post-infection (dpi) during different stages of symptom development. Results: We identified 624 differentially expressed genes (DEGs) in Arabidopsis influenced by RSV at 14 dpi and 21 dpi, among which at 14 dpi, 255 transcripts were induced, and 38 were repressed; at 21 dpi, 146 were induced, and 237 were repressed. Functional annotation indicated that these DEGs were related to multiple biological functions, including defense response, secondary metabolism, protein amino acid phosphorylation and response to abiotic stress. Conclusions: Importantly, the transcription of genes related to host defense systems was activated by RSV infection at an early stage of symptom development (14 dpi), whereas over the infection period (21 dpi), the host defense response systems were suppressed. A total of 52 genes were continuously differentially expressed between the two time points, indicating that the majority of DEGs were transient and unique to a particular time point during symptom development. The DEGs, particularly the defense response genes, identified in this study are candidates suitable for further functional analysis during the RSV-Arabidopsis interaction.
外文关键词：Rice stripe virus；RNA-seq；Digital gene expression (DGE)；Defense response
作者：Feng Sun；Peng Fang；Juan Li；Linlin Du；Ying Lan；Tong Zhou；Yongjian Fan； Wenbiao Shen；Yijun Zhou
作者单位：南京农业大学；江苏省农业科学院
期刊名称：virology journal
期刊影响因子：2.1389
出版年份：2016
出版刊次：12
点击下载：通过基于RNA测序的数字基因表达分析揭示了水稻条纹病毒在致病过程中对宿主防御反应的改变