中文摘要：转基因Bt水稻的出现为Bt作物、作物病原体和非靶标昆虫之间的互作创造了可能。尤其是关于病原体侵染的Bt表达植物将会如何影响非靶标昆虫方面的信息是预测GM种植制度可持续性所必需的。本研究开展了实验室生物测定以评估水稻矮缩病毒（RDV）和两种Bt水稻（T1C-19（Cry1C）和T2A-1 （Cry2A））对非靶标水稻叶蝉潜在的联合影响。根据研究结果，作者推断：这两种测试Bt水稻与RDV之间的交互影响并不会导致害虫种群扩大，由此表明，这两种Bt水稻品种对可持续水稻农业生态系统中的水稻叶蝉造成的风险微不足道。还需要大规模进行长期的监测GRLHs种群动态的田间试验验证该结论。
外文摘要：The advent of genetically modified (GM) Bt rice creates the possibility of interactions among Bt crops, crop pathogens and non-target herbivores. In particular, information on how pathogen-infected Bt-expressing plants will influence non-target herbivores is necessary to predict the sustainability of GM cropping systems. Laboratory bioassays were conducted to evaluate the potential combined impacts of rice dwarf virus (RDV) and two Bt rice lines, T1C-19 (Cry1C) and T2A-1 (Cry2A), on non-target green rice leafhopper (GRLH), Nephotettix cincticeps (Uhler) (Hemiptera: Cicadellidae). In the first experiment, GRLHs feeding preference tests on Bt rice lines compared to a parental control rice line, MH63, were conducted. As rice plants were uninfected with RDV, GRLHs generally preferred the control MH63 line over the two Bt lines during the initial 8 h, with no significant preference during the following 64 h. As rice plants were infected with RDV, there were no clear preferences between the Bt rice lines and the control MH63 line. In the second experiment, we assessed the combined influence of RDV-infection status and Bt rice lines on GRLH biological parameters. Egg duration, adult weights, and male adult longevity were significantly affected on RDV-infected Bt rice. Other parameters, egg hatching rate, nymph survival and fecundity were not significantly influenced. We infer that interaction effect among two testing Bt rice lines and RDV will not lead to enlarged pest populations, thus demonstrating that growing these two Bt rice lines will poses negligible risk to GRLH in sustainable rice agroecosystems. Long-term field experiments to monitor the population dynamics of GRLHs at large scale need to be carried out to confirm the current results.
作者：Wang, Qianjin; Han, Naishun; Dang, Cong; 等
作者单位：浙江大学
期刊名称：PLOS ONE
期刊影响因子：2.8059
出版年份：2017
出版刊次：7
点击下载：转基因Bt水稻和水稻矮缩病毒对非靶标昆虫黑尾叶蝉（半翅目：叶蝉科）生物学参数的综合影响研究