中文摘要：从甘薯粉虱（采集自巴西中西部（AdDF）与东南地区（AdO））上分离获得的两种新型小的环状DNA病毒全基因组序列是由下一代测序（NGS）决定的，可经克隆和Sanger测序确认。有2199和2211nt长的基因组分别编码假定的外壳蛋白（CP）和复制相关蛋白（Rep），且呈现出Genomoviridae属病毒典型的基因组结构。Rep推理的氨基酸序列系统发育分析表明，来自AdO的病毒与Gemycircularvirus属其他成员密切相关，而来自AdDF的病毒则与其他基因组病毒相关性较远。因此将其归类为假定的一个新属（建议将其称为"Gemybolavirus"）。这些新的genomoviruses暂时命名为“与烟粉虱相关的gemybolavirus AdDF”和“与烟粉虱相关的gemycircularvirus AdO”。
外文摘要：The complete genome sequences of two novel small circular DNA viruses isolated from sweet-potato whiteflies collected in central-West (AdDF) and Southeast (AdO) regions of Brazil were determined by Next Generation Sequencing (NGS), and confirmed by cloning and Sanger sequencing. The genomes are 2,199 and 2,211 nt-long, respectively, encoding a putative coat protein (CP) and a replication-associated protein (Rep) and showing a genomic organization typical of viruses from the family Genomoviridae. Phylogenetic analysis with deduced amino acid sequences of Rep indicates that the virus from AdO is closely related to other members of the genus Gemycircularvirus, while the virus from AdDF is distantly related to other genomovirus. It was thus classified in a putative new genus, for which the name "Gemybolavirus" is proposed. These new genomoviruses are tentatively named "Bemisia associated gemybolavirus AdDF", and "Bemisia associated gemycircularvirus AdO".
作者：Nakasu, Erich Y. T.; Melo, Fernando L.; Michereff-Filho, Miguel; Nagata, Tatsuya; Ribeiro, Bergmann M.; Ribeiro, Simone G.; Lacorte, Cristiano; Inoue-Nagata, Alice K.
作者单位：巴西Embrapa Vegetables
期刊名称：ARCHIVES OF VIROLOGY
期刊影响因子：2.058
出版年份：2017
出版刊次：9
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