中文摘要：遗传修饰（GM）的转基因水稻的出现为转基因作物、作物病原体和非靶标草食动物之间的交互作用创造了可能性。特别是，病原菌侵染的Bt表达植物将如何影响非靶标草食动物对于预测GM耕作系统的可持续性是很有必要的。本文开展了实验室生物测定，以评估水稻矮缩病毒（RDV）和两种Bt水稻（T1C-19 (Cry1C)和T2A-1 (Cry2A)）对非靶标水稻叶蝉（GRLH）潜在的综合影响。开展的第一个实验对比了GRLHs在Bt水稻品种和亲本对照水稻品种MH63上的取食偏好，结果发现，从未被RDV侵染的水稻来看，GRLHs在最初的8小时内通常偏好于取食对照水稻品种MH63胜过于两种Bt水稻，但随后的64小时内没有明显的差异；从被RDV侵染的水稻来看，GRLHs对Bt水稻和对照品种MH63没有明显的取食偏好差异。第二个实验评估了RDV侵染和Bt水稻品种对GRLH生物学参数的综合影响，结果发现，RDV侵染的Bt水稻上卵期、成虫体重和雄成虫的寿命受到显著影响；卵孵化率、蛹存活率和繁殖力均未受显著影响。作者推断两种测试的Bt水稻与RDV之间的相互影响不会导致害虫种群扩大，由此表明种植这两种Bt水稻品种将对可持续水稻农业生态系统中GRLH造成的风险很小，可忽略不计。
外文摘要：The advent of genetically modified (GM) Bt rice creates the possibility of interactions among Bt crops, crop pathogens and non-target herbivores. In particular, information on how pathogen-infected Bt-expressing plants will influence non-target herbivores is necessary to predict the sustainability of GM cropping systems. Laboratory bioassays were conducted to evaluate the potential combined impacts of rice dwarf virus (RDV) and two Bt rice lines, T1C-19 (Cry1C) and T2A-1 (Cry2A), on non-target green rice leafhopper (GRLH), Nephotettix cincticeps (Uhler) (Hemiptera: Cicadellidae). In the first experiment, GRLHs feeding preference tests on Bt rice lines compared to a parental control rice line, MH63, were conducted. As rice plants were uninfected with RDV, GRLHs generally preferred the control MH63 line over the two Bt lines during the initial 8 h, with no significant preference during the following 64 h. As rice plants were infected with RDV, there were no clear preferences between the Bt rice lines and the control MH63 line. In the second experiment, we assessed the combined influence of RDV-infection status and Bt rice lines on GRLH biological parameters. Egg duration, adult weights, and male adult longevity were significantly affected on RDV-infected Bt rice. Other parameters, egg hatching rate, nymph survival and fecundity were not significantly influenced. We infer that interaction effect among two testing Bt rice lines and RDV will not lead to enlarged pest populations, thus demonstrating that growing these two Bt rice lines will poses negligible risk to GRLH in sustainable rice agroecosystems. Long-term field experiments to monitor the population dynamics of GRLHs at large scale need to be carried out to confirm the current results.
作者：Wang, Qianjin; Han, Naishun; Dang, Cong; 等
作者单位：浙江大学
期刊名称：PLOS ONE
期刊影响因子：2.8059
出版年份：2017
出版刊次：7
点击下载：转Bt基因水稻和水稻矮缩病毒对非靶标草食动物水稻黑尾叶蝉生物学参数的综合影响研究