中文摘要：在植物中，病毒诱导的基因沉默（VIGS）是进行功能基因组研究或快速评价个体基因功能时比较受欢迎的一种工具。然而，关于植物对病毒载体响应的分子机制仍不清楚，这可能会使实验设计和数据解释复杂化。因此，本文通过全面的全基因组分析，记录了番茄因为使用最为广泛的烟草脆裂病毒（TRV）载体而引起的整个转录组的变化。实验数据阐明了多重生物进程与功能影响，最重要的是，研究发现了Mrna选择性剪接模式的大规模变化。这些结果有助于将来VIGS载体在功能研究中的应用（对植物研究群体有益），并有助于加深对植物-病毒交互作用的理解。
外文摘要：In plants, virus-induced gene silencing (VIGS) is a popular tool for functional genomic studies or rapidly assessing individual gene functions. However, molecular details regarding plant responses to viral vectors remain elusive, which may complicate experimental designs and data interpretation. To this end, we documented whole transcriptome changes of tomato elicited by the application of the most widely used tobacco rattle virus (TRV)-based vectors, using comprehensive genome-wide analyses. Our data illustrated multiple biological processes with functional implications, including (1) the enhanced activity of miR167 in guiding the cleavage of an auxin response factor; (2) reduced accumulation of phased secondary small interfering RNAs from two genomic loci; (3) altered expression of similar to 500 protein-coding transcripts; and (4) twenty long noncoding RNAs specifically responsive to TRV vectors. Importantly, we unraveled large-scale changes in mRNA alternative splicing patterns. These observations will facilitate future application of VIGS vectors for functional studies benefiting the plant research community and help deepen the understanding of plant-virus interactions.
作者：Zheng, Yi; Ding, Biao; Fei, Zhangjun; 等
作者单位：康奈尔大学
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.259
出版年份：2017
出版刊次：8
点击下载：全面的转录组分析揭示了番茄对烟草脆裂病毒基因沉默载体的反应