中文摘要：本研究从农田里收集了具有明显的黄脉花叶症状的和健康的没有任何病毒症状的黄秋葵叶片样本。通过聚合酶链反应（PCR）和对扩增产物进行克隆和测序证实了被感染病毒样本中菜豆金黄花叶病毒属的存在。基因组分析显示，在目前研究中隔离种群与贝因迪黄脉花叶病毒(BYVMV)有99%的核苷酸一致性，由此认为该病毒是BYVMV的变种。田间收集的烟粉虱经鉴定分为Asia-1和MEAM-1两种遗传种类。烟粉虱两种遗传种类的病毒-寄主关系比较表明了在MEAM-1和Asia-1遗传种类中至少有2到3种烟粉虱是每种植物传播病毒所需要的。传播YVMD病毒所需要的最短的获取周期和接种周期为15（MEAM1）和20分钟（Asia-1），并通过BYVMV外壳蛋白基因特异性探测核酸杂交进一步证实。
外文摘要：The leaf sample from okra plants showing prominent yellow vein mosaic symptoms and healthy plant without any virus symptoms were collected from farmer's field. The presence of begomovirus in the infected sample was confirmed by polymerase chain reaction (PCR) and the amplicons were cloned and sequenced. The genome analysis showed that the isolate in the present study had 99% nucleotide identity with Bhendi yellow vein mosaic virus (BYVMV) revealing it as BYVMV variant. The genetic species of Bemisia tabaci collected from fields were identified as Asia-1 and MEAM-1 genetic species based on silver leaf assay, sequence characterized amplified region marker, and mtCOI gene sequence. The comparative virus-vector relationship of both genetic species of B. tabaci indicates a minimum of two and three B. tabaci in MEAM-1 and Asia-1 genetic species, respectively, per plant were required to transmit the disease. The minimum acquisition access period and inoculation access period of 15 (MEAM1) and 20 min (Asia-1) were required to transmit the YVMD; it was further confirmed by nucleic acid hybridization using coat protein gene-specific probe of BYVMV. With respect to the sex, the female B. tabaci were more efficient in transmitting the disease as compared to male ones in both the genetic species of B. tabaci. The MEAM-1 to transmit the BYVMV more efficiently than Asia-1 genetic species of B. tabaci.
外文关键词：Bhendi yellow vein mosaic virus; Bemisia tabaci; Genetic species; Transmission
作者：Venkataravanappa, V.; Kodandaram, M. H.; Reddy, C. N. Lakshminarayana; 等
作者单位：ICAR Inst Hort Res
期刊名称：3 BIOTECH
期刊影响因子：1.361
出版年份：2017
出版刊次：9