中文摘要：新德里番茄曲叶病毒（ToLCNDV）是一种自2012年以来严重影响西班牙甜瓜主产区甜瓜生长的双生菜豆金黄花叶病毒属病毒。本研究评估了四份资源及其对应杂交种的抗性水平。研究发现了控制ToLCNDV抗性的三个基因组区域，一个主要位于11号染色体，还有两个分别位于12号染色体和2号染色体。最高水平抗性是从11号染色体QTL纯合基因型WM-7WM-7中甚至是其他两个位点PSPS基因型中获得的。从WM-7中获得的抗性有助于开发甜瓜新品种，本文中筛选出来的相连的SNPs对甜瓜ToLCNDV抗性分子标记辅助育种是非常有用的。
外文摘要：Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite begomovirus that severely affects melon crop (Cucumis melo) in the main production areas of Spain since 2012. In this work, we evaluated the degree of resistance of four accessions (two belonging to the subsp. agrestis var. momordica and two to the wild agrestis group) and their corresponding hybrids with a susceptible commercial melon belonging to the subsp. melo (Piel de Sapo, PS). The analysis using quantitative PCR (qPCR) allowed us to select one wild agrestis genotype (WM-7) with a high level of resistance and use it to construct segregating populations (F2 and backcrosses). These populations were phenotyped for symptom severity and virus content using qPCR, and genotyped with different sets of SNP markers. Phenotyping and genotyping results in the F2 and BC1s populations derived from the WM-7 × PS cross were used for QTL analysis. Three genomic regions controlling resistance to ToLCNDV were found, one major locus in chromosome 11 and two additional regions in chromosomes 12 and 2. The highest level of resistance (no or mild symptoms and very low viral titer) was obtained with the homozygous WM-7WM-7 genotype at the major QTL in chromosome 11, even with PSPS genotypes at the other two loci. The resistance derived from WM-7 is useful to develop new melon cultivars and the linked SNPs selected in this paper will be highly useful in marker-assisted breeding for ToLCNDV resistance in melon.
外文关键词：ToLCNDV；Cucumis melo；Resistance；Genetic control；Breeding；Linked SNPs
作者：Saez, Cristina；Esteras, Cristina；Martinez, Cecilia；等
作者单位：西班牙瓦伦西亚理工大学
期刊名称：Plant Cell Reports
期刊影响因子：2.8689
出版年份：2017
出版刊次：10
点击下载：11号染色体QTL对甜瓜新德里番茄曲叶病毒抗性的控制研究