中文摘要：番茄斑萎病毒（TSWV）非结构蛋白（NSs）已被确定为辣椒Tsw抗性的无毒因素。匈牙利野生型（WT）和抗性破坏型（RB）TSWV分离株的NSs只有两个氨基酸替代（104，461）。本文分析了补丁试验中NSs及其点突变变种触发Tsw介导的过敏反应和RNA沉默抑制子活性的能力。研究确定了位点104（T-A）上一个单一的氨基酸变化，主要负责坏死感应或损失。本研究结果直接表明了单个氨基酸的变化可以诱导RB表型。
外文摘要：The nonstructural protein (NSs) of Tomato spotted wilt virus (TSWV) was previously identified as an avirulence determinant for Tsw-based resistance on pepper. The NSs of wild-type (WT) and resistance-breaking (RB) TSWV strains isolated in Hungary had only two amino acid substitutions (104, 461). We have analysed the ability of the NSs and their point mutant variants to trigger Tsw-mediated hypersensitive responses and RNA silencing suppressor (RSS) activity in patch assays. We identified a single amino acid change at position 104 (T-A) that was responsible for the necrosis induction or loss, white a significant difference was not detected in the RSS activity of the two parental strains. We have successfully complemented the infection of the WT strain on resistant pepper cultivar with the infectious S RNA transcript of the RB strain and the WT-T104A point mutant. Our work provides direct evidence that a single amino acid change can induce an RB phenotype.
外文关键词：TSWV；NSs；resistance breaking；Tsw gene；transient expression；complementation
作者：Almasi, Aszteria；Nemes, Katatin；Csomor, Zsofia；等
作者单位：匈牙利科学院
期刊名称：JOURNAL OF GENERAL VIROLOGY
期刊影响因子：2.838
出版年份：2017
出版刊次：6