中文摘要：病毒侵染影响着植物miRNA的表达模式。已有推测，miR171和其他一些miRNAs的减少会影响植物病毒症状。本研究通过试验证明osa-miR171b与水稻上水稻条纹病毒（RSV）症状之间的关联。抑制miR171导致了有相似病毒症状的叶片中叶绿素含量减少，并伴随矮缩。通过人工miRNA的osa-miR171b超表达促进了植物的生长，增强了叶片中叶绿素的积累。与对照相比，osa-miR171b超表达的植株分蘖变厚，小穗变多，穗变长，但分蘖数没有显著差异。总的来说，研究结果表明，被RSV侵染的水稻中osa-miR171b的减少有助于RSV症状的显现，这为研究osa-miR171b在水稻中的作用提供了更多的视角。
外文摘要：Viral infection affects the pattern of plant miRNA expression. It has been presumed that reduction of miR171 and several other miRNAs influences viral symptoms in plants. We here experimentally demonstrate the association of osa-miR171b with rice stripe virus (RSV) symptoms in rice. Inhibition of osa-miR171b caused stunting with reduced chlorophyll content in leaves similar to viral symptoms. Overexpression of osa-miR171b by an artificial miRNA extended vegetative growth and enhanced chlorophyll accumulation in leaves. Tillers were thicker, and panicles were longer with more spikelets in plants overexpressing osa-miR171b than in controls, but there were no differences in tiller numbers. Targets of osa-miR171b, OsSCL6-IIa, OsSCL6-IIb, and OsSCL6-IIc, were respectively up-and downregulated in plants where osa-miR171b was inhibited or overexpressed. In plants overexpressing osa-miR171b, five positive regulators for heading development, Ehd1, Ehd2, Ehd3, Ehd4, and Hd3a were up-regulated, while the negative regulator Ghd7 was down-regulated. Plants overexpressing osa-miR171b were less susceptible to RSV and virus symptoms were attenuated. Taken together, the results reveal that a reduction of osa-miR171b in RSV-infected rice contributes to RSV symptoms, and provide more insight into the roles of osa-miR171b in rice.
外文关键词：Early heading date genes；Ehd；Hd3a；heading；miR171；SCARECROW-LIKE6； SCL6；symptoms；reproductive growth；rice stripe virus；vegetative growth
作者：Tong, Aizi；Yuan, Quan；Wang, Shu；等
作者单位：浙江省农业科学院
期刊名称：JOURNAL OF EXPERIMENTAL BOTANY
期刊影响因子：4.4149
出版年份：2017
出版刊次：7
点击下载：osa-miR171b改变的积累可调控病害症状，有助于水稻条纹病毒的入侵