中文摘要：植物的植食性防御系统主要是由茉莉酸（JA）和水杨酸（SA）信号通路介导的。这样的防御机制可能会影响昆虫取食动态，可能还会影响病毒、植物与介体昆虫之间的传播-获取关系。在番茄-烟粉虱-番茄黄化曲叶病毒（TYLCV）生物学模型背景下，本文研究测试了非介体昆虫（桃蚜）先侵染植株对介体昆虫（烟粉虱）取食动态和病毒传播-获取的影响。研究结果表明，蚜虫先侵染对番茄-烟粉虱- TYLCV系统有显著的影响。TYLCV的传播和获取与烟粉虱取食活动呈正相关，且均由SA和JA信号通路介导的。在传播阶段，TYLCV浓度分别由水杨酸上调、茉莉酸下调。在获取阶段，这两种通路不一致；SA相关基因上调，而JA通路上下游则表现出更复杂的关系。植物-植食性动物-病毒交互作用对生态健康的昆虫和病原菌管理计划具有潜在的重要意义，本研究这些发现增强了对三者之间交互作用的理解。
外文摘要：Herbivory defense systems in plants are largely regulated by jasmonate-(JA) and salicylate-(SA) signaling pathways. Such defense mechanisms may impact insect feeding dynamic, may also affect the transmission-acquisition relationship among virus, plants and vectoring insects. In the context of the tomato - whitefly - Tomato Yellow Leaf Curl Virus (TYLCV) biological model, we tested the impact of pre-infesting plants with a non-vector insect (aphid Myzus persicae) on feeding dynamics of a vector insect (whitefly Bemisia tabaci) as well as virus transmission-acquisition. We showed that an aphid herbivory period of 0-48 h led to a transient systemic increase of virus concentration in the host plant (root, stem, and leaf), with the same pattern observed in whiteflies feeding on aphid-infested plants. We used real-time quantitative PCR to study the expression of key genes of the SA-and JA-signaling pathways, as well as electrical penetration graph (EPG) to characterize the impact of aphid pre-infestation on whitefly feeding during TYLCV transmission (whitefly to tomato) and acquisition (tomato to whitefly). The impact of the duration of aphid pre-infestation (0, 24, or 48 h) on phloem feeding by whitefly (E2) during the transmission phase was similar to that of global whitefly feeding behavior (E1, E2 and probing duration) during the acquisition phase. In addition, we observed that a longer phase of aphid pre-infestation prior to virus transmission by whitefly led to the up-regulation and down-regulation of SA-and JA-signaling pathway genes, respectively. These results demonstrated a significant impact of aphid pre-infestation on the tomato - whitefly - TYLCV system. Transmission and acquisition of TYLCV was positively correlated with feeding activity of B. tabaci, and both were mediated by the SA-and JA-pathways. TYLCV concentration during the transmission phases was modulated by up- and down-regulation of SA- and JA-pathways, respectively. The two pathways were inconsistent during the acquisition phase; SA- related genes were up-regulated, whereas those up- and down-stream of the JA pathway showed a more complex relationship. These findings enhance our understanding of plant - herbivore - virus interactions, which are potentially important for development of ecologically sound pest and pathogen management programs.
外文关键词：TaqMan Real-Time PCR；systemic induced defense；Bemisia tabaci；Myzus persicae；electrical penetration graph；jasmonic acid；salicylic acid；signaling pathways
作者：Tan, Xiao L.; Chen, Ju L.; Benelli, Giovanni; 等
作者单位：中国科学院
期刊名称：FRONTIERS IN PLANT SCIENCE
期刊影响因子：4.2984
出版年份：2017
出版刊次：9
点击下载：番茄先遭蚜虫侵染对烟粉虱、番茄黄化曲叶病毒（TYLCV）和植株之间传播-获取关系的调节作用研究