中文摘要：水稻黑条矮缩病毒（RBSDV）是呼肠孤病毒科斐济病毒属成员之一，可对中国及许多其他亚洲国家的水稻生产造成显著的经济损失。虽然为了阐明病毒、昆虫介体、宿主和环境条件之间的交互作用已做了很多努力，但与发病机制相关的RBSDV蛋白很少有被确认，而且水稻病害发展的生物学基础仍是未知。与水稻病害发展相关的转录组学信息将有助于阐明生物学机制。为了确定水稻转录组如何变化以响应RBSDV侵染，本文通过RNA测序对易感水稻品种KTWYJ3进行了全基因组基因表达分析。分析表明，响应RBSDV侵染时，一些与病害防御和胁迫抗性相关的基因会上调，而与叶绿体相关的基因则会下调。此外，有些与植株高度相关的基因会差异表达。研究结果表明，这些基因可能与RBSDV引起的矮缩症状有关。总之，本研究结果提供了水稻响应RBSDV侵染时的全基因组转录组分析，这有助于理解水稻-RBSDV交互作用的调控机制以及水稻黑条矮缩病在水稻中发展的生物学基础。
外文摘要：Rice black-streaked dwarf virus (RBSDV), a member of the genus Fijivirus in the family Reoviridae, causes significant economic losses in rice production in China and many other Asian countries. Although a great deal of effort has been made to elucidate the interactions among the virus, insect vectors, host and environmental conditions, few RBSDV proteins involved in pathogenesis have been identified, and the biological basis of disease development in rice remains largely unknown. Transcriptomic information associated with the disease development in rice would be helpful to unravel the biological mechanism. To determine how the rice transcriptome changes in response to RBSDV infection, we carried out RNA-Seq to perform a genome-wide gene expression analysis of a susceptible rice cultivar KTWYJ3. The transcriptomes of RBSDV-infected samples were compared to those of RBSDV-free (healthy) at two time points (time points are represented by group I and II). The results derived from the differential expression analysis in RBSDV-infected libraries vs. healthy ones in group I revealed that 102 out of a total of 281 significant differentially expressed genes (DEGs) were up-regulated and 179 DEGs were down-regulated. Of the 2592 identified DEGs in group II, 1588 DEGs were up-regulated and 1004 DEGs were down-regulated. A total of 66 DEGs were commonly identified in both groups. Of these 66 DEGs, expression patterns for 36 DEGs were similar in both groups. Our analysis demonstrated that some genes related to disease defense and stress resistance were up-regulated while genes associated with chloroplast were down-regulated in response to RBSDV infection. In addition, some genes associated with plant-height were differentially expressed. This result indicates those genes might be involved in dwarf symptoms caused by RBSDV. Taken together, our results provide a genome-wide transcriptome analysis for rice plants in response to RBSDV infection which may contribute to the understanding of the regulatory mechanisms involved in rice-RBSDV interaction and the biological basis of rice black-streaked dwarf disease development in rice.
外文关键词：Rice black-streaked dwarf disease; RNA-Seq; Rice; Transcriptome analysis
作者：Ahmed, Mohamed M. S.; Ji, Wen; Wang, Muyue; 等
作者单位：扬州大学
期刊名称：GENE
期刊影响因子：2.4149
出版年份：2017
出版刊次：9
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