中文摘要：本文对感染茄子斑驳矮化病毒（EMDV）的番茄叶片中抗坏血酸及其生物合成路径基因表达变化进行了研究。研究结果表明，番茄和EMDV之间的交互作用可影响抗坏血酸的质量及其在不同番茄生态型植株发育阶段和果实成熟阶段的累积。
外文摘要：Changes in ascorbic acid levels and gene expression of its biosynthetic pathway were studied in Solanwn lycopersicum plants inoculated with Eggplant Mottled Dwarf Virus (EMDV). Data showed that EMDV inoculation caused a significant decrease of tAsA in the leaves of two ecotypes at 7 dpi, before symptom appearance, and at 21 dpi when symptoms were most evident and was restored at 35 dpi when symptoms remained constant. Biosynthetic gene expression decreased at 7 and 21 dpi. In Tondo Giallo the tAsA levels were restored at 35 dpi; which coincided with an upregulation of almost all genes. In immature fruits of Maiese, the decrease of tAsA was accompanied by a substantial down-regulation of genes in L-galactose and/or L-galactonic acid pathways while in Tondo Giallo the constant level of tAsA was associated with an increase of ASA and with an upregulation of gene expression. This could be a direct consequence of a high ascorbate turnover and a low level of oxidative stress. In ripe fruits, the higher tAsA content observed in Maiese was associated with a general upregulation of gene expression, while in Tondo Giallo it could depend on a high rate of ascorbate recycling as suggested by the upregulation of the gene MDHAR2. Collectively these results provide evidence that the interaction between Solanum lycopersicon and EMDV affects ascorbic acid quality and accumulation in relation to tomato ecotype, plant developmental stage, and fruit ripening stage.
外文关键词：EMDV; Antioxidant; Vitamin C; Secondary metabolite; Ascorbic acid; Ascorbate
作者：Zarrillo, A.; Minutolo, M.; Alioto, D.; 等
作者单位：那不勒斯菲里德里克第二大学
期刊名称：SCIENTIA HORTICULTURAE
期刊影响因子：1.6239
出版年份：2017
出版刊次：11
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