中文摘要：乙烯在植物发育和生物胁迫反应中起着关键作用，但乙烯在寄主抗病毒反应中的作用机制仍是未知。本文研究发现，水稻矮缩病毒（RDV）通过激活S-腺苷甲硫氨酸合成酶（SAMS，乙烯合成途径中的关键成分）活性促进了乙烯的产生，可导致对RDV的敏感性有所提高。研究结果揭示了RDV操控寄主体内乙烯的生物合成从而实现有效侵染的一个新机制。
外文摘要：Ethylene plays critical roles in plant development and biotic stress response, but the mechanism of ethylene in host antiviral response remains unclear. Here, we report that Rice dwarf virus (RDV) triggers ethylene production by stimulating the activity of S-adenosyl-L-methionine synthetase (SAMS), a key component of the ethylene synthesis pathway, resulting in elevated susceptibility to RDV. RDV-encoded Pns11 protein specifically interacted with OsSAMS1 to enhance its enzymatic activity, leading to higher ethylene levels in both RDV-infected and Pns11-overexpressing rice. Consistent with a counter-defense role for ethylene, Pns11-overexpressing rice, as well as those overexpressing OsSAMS1, were substantially more susceptible to RDV infection, and a similar effect was observed in rice plants treated with an ethylene precursor. Conversely, OsSAMS1-knockout mutants, as well as an osein2 mutant defective in ethylene signaling, resisted RDV infection more robustly. Our findings uncover a novel mechanism which RDV manipulates ethylene biosynthesis in the host plants to achieve efficient infection.
作者：Zhao, Shanshan; Hong, Wei; Wu, Jianguo; 等
作者单位：北京大学
期刊名称：ELIFE
期刊影响因子：7.725
出版年份：2017
出版刊次：10
点击下载：病毒蛋白对寄主SAMS1活性和乙烯产生的促进作用有利于病毒的侵染