中文摘要：RNA干扰（RNAi）是植物和其他生物抵抗病毒侵染的关键防御机制之一。在植物被病毒侵染的情况下，短干扰RNAs（siRNAs）由RNA病毒分子双链复制形式构成。这些siRNAs是RNAi的基本组成之一，RNA诱导的基因沉默复合体（RISC）与移除病毒RNA的特定序列有关。病毒蛋白P19是RNAi的抑制子，能够在siRNAs与RISC相结合前阻止siRNAs的形成。本研究证实了，病毒siRNAs的初步加入通过早期计划好的RISC和RNAi防御活性的激活提高了植物对病毒侵染的耐受性。
外文摘要：RNA interference (RNAi) is one of the key defense mechanisms directed against virus infections in plants and other organisms. In this case in plants infected with viruses, short interfering RNAs (siRNAs) are formed from two-chain replicated forms of virus molecules of RNA. These siRNAs program one of the RNAi basic components, RNA-induced complex of genes silencing (RISC, RNA induced silencing complex) associated with sequence-specific removing virus RNA. Virus protein P19 is a suppressor of RNAi and is capable of trapping the siRNAs being formed before their binding with RISC. Here, it was shown that preliminary entering leaves of plants Nicotiana benthamiana Domin (before virus infecting) of siRNAs eluted from the complex P19/siRNA from the infected plant lowers development of infection symptoms induced by tomato bushy stunt virus (TBSV) in inoculated plants. Exogenous addition of suppressor-associated siRNAs to plants leads to not only lowering virus accumulation but also to survival of infected plants. Thus, it has been established that preliminary addition of virus siRNAs elevates plant tolerance to the virus infection by means of early programming RISC and activation of the defense action of RNAi.
外文关键词：Nicotiana benthamiana; RNA interference; siRNA; gel chromatography; complex P19/siRNA
作者：Sutula, M. Yu.; Akbassova, A. Zh.; Yergaliyev, T. M.; 等
作者单位：哈萨克斯坦国立欧亚大学
期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
期刊影响因子：0.739
出版年份：2017
出版刊次：11
点击下载：通过短干扰RNAs的初步处理使植物具有抵抗病毒侵染的耐受性