中文摘要：番茄黄化曲叶病是由菜豆金色花叶病毒属病毒引起的，可对番茄生产造成严重威胁。如果菜豆金色花叶病毒属病毒（由烟粉虱传播）在番茄生长初期就侵染番茄，则会使番茄生产完全损失。北碧番茄曲叶病毒（TYLCKaV）的出现对东南亚茄属作物的生产造成了严重的威胁。有些菜豆金色花叶病毒属的机械性传播的缺乏是实验的主要限制。本研究建立了一种番茄TYLCKaV农杆菌接种法，这种方法有助于简单、快速评估番茄对TYLCKaV的抗性。
外文摘要：Tomato yellow leaf curl disease caused by begomoviruses is a serious threat to tomato (Solanum lycopersicum L.) production. If begomoviruses, transmitted by whitefly (Bemisia tabaci), infect tomato plants during early growth, production can be almost entirely lost. Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), a bipartite Begomovirus, is emerging as an important threat to solanaceous crop production in Southeast Asia. The lack of mechanical transmission of some begomoviruses is a major experimental constraint. In this study, an agroinoculation method using TYLCKaV in tomato plants was established. Partial tandem repeats of TYLCKaV DNA A and DNA B were constructed and cloned to a binary pGreenII vector, and their infectivity was tested. Co-inoculation of TYLCKaV DNA A and DNA B to Nicotiana benthamiana L. produced typical begomoviral symptoms, and both of the viral DNA components accumulated in the upper uninoculated leaves, suggesting systemic infection of TYLCKaV. Two agroinoculation methods were conducted on tomatoes. First, excised sections of tomato shoots were agroinoculated with a soaking procedure. Although two Agrobacterium tumefaciens strains were tested, approximately 40% of inoculated plants only showed viral symptoms for EHA105. Second, agrobacterium from a cultured petri dish was directly inoculated with a colony inoculation procedure. When EHA105 was used, approximately 92% of inoculated plants showed viral symptoms. Sequencing the recovered viral DNA from the upper uninoculated leaf clarified that TYLCKaV had successfully infected the tomato plants. The colony inoculation procedure is labor-saving, and viral symptoms develop in susceptible tomato plants within approximately a month from sowing the seeds. This method could contribute to simple and speedy evaluation of TYLCKaV resistance of tomato plants.
外文关键词：begomovirus; geminivirus; infectious clone; Southeast and East Asia; TYLCKaV
作者：Koeda, Sota; Homma, Kanami; Tanaka, Yuri; 等
作者单位：Kindai University (Kinki University)
期刊名称：HORTICULTURE JOURNAL
期刊影响因子：0.683
出版年份：2017
出版刊次：1
点击下载：一种高效接种北碧番茄曲叶病毒的农杆菌接种法