中文摘要：黄瓜花叶病毒（CMV）是番茄病害中最重要的病原之一。有些CMV病毒株有助于病毒复制、衣壳化和卫星RNAs（satRNA）的传播，可能会加剧某些寄主上CMV引起的症状。CMV毒株（支持satRNAs高毒力变异型）的爆发常会在番茄上反复发生，对作物生产造成了毁灭性的影响，但仍未有有效的管理方法。本文研究了CMV毒株TTS和77的侵染动态，它们可在Sl-Ma和Sl-UC两个番茄品种上共同作用导致植株顶端矮化（TTS-satRNA）和坏死（77-satRNA）。虽然这两个品种都屈从于CMV-77及其坏死satRNA，但是Sl-Ma可从CMV-TTS引发的病害症状中恢复，而Sl-UC不可以恢复。Sl-Ma植株的恢复能力可以通过嫁接传输至易感基因型Sl-UC上。对小RNAs和基于RNA干扰（RNAi）的植物防御反应基因的分析表明，RNAi参与了Sl-Ma对抗高毒satRNAs CMV的恢复。
外文摘要：Cucumber mosaic virus (CMV) lists among the most important etiological agents of tomato diseases. Some isolates of CMV function as helper virus for replication, encapsidation and transmission of satellite RNAs (satRNA), which may exacerbate symptoms induced by CMV in certain hosts. Outbreaks of CMV strains supporting hypervirulent variants of satRNAs are recurrent in tomato with devastating effects on crop production and efficient control measures are still unavailable. In this study, we examined the dynamics of infection of the CMV strains tomato top stunting (TTS) and 77 supporting replication of satRNA variants that codetermine top stunting (TTS-satRNA) and necrotic (77-satRNA) phenotypes in two tomato cultivars denoted Solanumlycopersicum Manduria (Sl-Ma) and S. lycopersicum UC82 (Sl-UC). Sl-Ma but not Sl-UC recovered from disease symptoms induced by CMV-TTS while both the cultivars succumbed to the infection of CMV-77 and its necrogenic satRNA. Ability to recover of the Sl-Ma plants was transmitted by grafting to the susceptible genotype Sl-UC. More interestingly, recovery was observed also against the challenge inoculation of CMV plus 77-satRNA in plants grafted on Sl-Ma and in self-grafted plants of both the Sl-Ma and Sl-UC cultivars. Analysis of small RNAs and genes of the defence plant response based on RNA interference (RNAi) suggested that RNAi is involved in the recovery of Sl-Ma against CMV with hypervirulent satRNAs and in scions grafted on this rootstock. The response of Sl-Ma to the inoculation of CMV-77 plus 77-satRNA was compared with that of the transgenic tomato line S. lycopersicum transgenic line UCTC5.9.2 that expresses constitutively the benign variant of the satRNA denoted Tfn-satRNA. Comparative analysis suggested that the response may operate via similar mechanisms, which involve RNAi, the graft and the presence of the satRNA.
外文关键词：Cucumber mosaic virus; grafting; recovery in grafted plants; satellite RNA
作者：Spano, R.; Gallitelli, D.; Mascia, T.
作者单位：巴里大学
期刊名称：ANNALS OF APPLIED BIOLOGY
期刊影响因子：2.046
出版年份：2017
出版刊次：11
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