中文摘要：在印度大豆主产区的印度中部，豆类作物可能会遭受由绿豆黄花叶病毒（MYMIV）引起的黄花叶病。MYMIV是由烟粉虱传播的，烟粉虱是一个由多种不同基因组组成的复合种群。该介体种类可在区域菌株和个体间藏有不同的内共生体。为了阐明中央邦（印度中部的一个邦）该病毒介体的基础方面，作者对烟粉虱体内共生菌和病毒的侵染状态进行了研究。对搜集自中央邦的烟粉虱进行聚合酶链反应（PCR）分析，除发现了初级内共生菌Portiera外，还发现了4种次生内共生菌，即Arsenophonus、Hemipteriphilus、Wolbachia和Cardinium。Arsenophonus和Hemipteriphilus高度侵染，但Wolbachia和Cardinium的侵染速率较低。在搜集自中央邦不同寄主植物上的烟粉虱种群内检测出了MYMIV。在中央邦种群中并未观察到共生体侵染状态、病毒侵染和烟粉虱基因组之间有特定的关系。研究设计的新引物，可用于PCR模板的验证以及识别两种密切的系统内共生体。
外文摘要：Legume crops in Central India, the main soybean production area of the country, may suffer from yellow mosaic disease caused by the Mungbean yellow mosaic India virus (MYMIV). MYMIV is transmitted by the sweet potato whitefly, Bemisia tabaci (Gennadius), which is a species complex composed of various genetic groups. This vector species harbors different endosymbionts among regional strains and among individuals. To elucidate fundamental aspects of this virus vector in the state of Madhya Pradesh, the infection status of the symbionts and the virus in whiteflies was studied. A polymerase chain reaction (PCR) survey of the whiteflies collected in Madhya Pradesh found four secondary endosymbionts, Arsenophonus, Hemipteriphilus, Wolbachia, and Cardinium, in addition to the primary endosymbiont Portiera. Arsenophonus and Hemipteriphilus were highly infected but the infection rates of Wolbachia and Cardinium were low. MYMIV was detected in whitefly populations collected from various host plants in Madhya Pradesh. The whitefly populations belonged to the Asia I and II genetic groups; several different Asia II populations were also distributed. Specific relations were not observed among symbiont infection status, virus infection, and the whitefly genetic groups in the populations of Madhya Pradesh, though Cardinium was highly detected in the Asia II-1 group. New primers, which can be used for PCR template validation and for discriminating two phylogenetically close endosymbionts, were designed.
外文关键词：Legume crop; Yellow mosaic disease; Endosymbionts; Vector species; Soybean 期刊名称：APPLIED ENTOMOLOGY AND ZOOLOGY
作者：Ansari, Parveen G.; Singh, Rakesh K.; Kaushik, Shruti; 等
作者单位：印度RVSKVV
期刊名称：APPLIED ENTOMOLOGY AND ZOOLOGY
期刊影响因子：0.888
出版年份：2017
出版刊次：11
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