中文摘要：已有研究证明RNA依赖的RNA聚合酶1（RDR1）参与了DNA甲基化、RNA沉默和调节其他基因的表达。马铃薯Y病毒（PVY）的侵染会刺激RDR1基因的表达。RDR1基因表达沉默的转基因烟草表现出叶肉细胞（与核重塑有关）、叶绿体和线粒体的形态学变化。RDR1沉默导致核变小、异染色质的含量和聚集增加、叶绿体的数量减少，且叶绿体和线粒体的外形、内在结构和完整性发生了变化。RDR/-沉默的转基因植株表现出PVY积累增多，且强化了遭PITY侵染的RDR/-沉默植株的叶绿体和线粒体超微结构的重塑。因此，RDR1可调节基因表达，引起染色体重塑，而PVY侵染抵消了对染色体重塑的这些影响。
外文摘要：RNA-dependent RNA polymerase 1 (RDR1) has been shown to be involved in DNA methylation, RNA silencing and regulating expression of other genes. RDR1 gene expression is stimulated by infection with potato virus Y degrees (PVY). Transgenic Nicotiana tabacum plants silenced for RDR1 gene expression showed morphological changes in mesophyll cells, associated with remodeling of the nuclei, chloroplasts and mitochondria. RDR1 silencing led to decreased nuclear size, increased heterochromatin content and aggregation, decreased numbers of chloroplasts, plus changes in shape, internal structures and integrity of chloroplasts and mitochondria. RDR/-silenced transgenic plants showed increased PVY accumulation and ultrastructural remodeling was intensified in both chloroplasts and mitochondria of PITY-infected, RDR/-silenced plants. By contrast, heterochromatin condensation was reduced by PVY infection, and in non-transgenic plants the nuclei were translucent and lacked morphology after PVY infection. Thus, RDR1 regulates gene expression leading to remodeling of chromosomes, and PVY infection counteracts these effects on chromosomal remodeling.
外文关键词：RNA-dependent RNA polymerase; Potato virus Y; Heterochromatin; Euchromatin; Nuclear remodeling; Chromosomal remodeling; Organelle remodeling
作者：Rakhshandehroo, Farshad; Rezaee, Saeed; Palukaitis, Peter
作者单位：首尔女子大学
期刊名称：VIROLOGY
期刊影响因子：3.353
出版年份：2017
出版刊次：10
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