番茄黄化曲叶病毒（TYLCV）可使番茄生产遭受巨大损失。除了番茄，TYLCV可侵染很多作物或杂草作为其可替代的寄主。这些替代寄主可作为TYLCV存活和传播的库源。本研究测试了烤烟作为TYLCV寄主的能力。在九种烤烟上接种了传染性TYLCV克隆后检测其TYLCV DNA，发现在感染了TYLCV的烟草上并没有明显的症状。携带病毒DNA的烟粉虱百分比迅速增长，并于12小时后达到100%。以侵染的烟草为病毒源，TYLCV可由烟粉虱传播至烟草和番茄上，在侵染后的番茄上可以观察到TYLCV侵染的典型症状，但在侵染的烟草上没有观察到症状。这些研究结果表明，烤烟可以作为TYLCV的寄主植物，在中国TYLCV流行和传播中起着重要的作用。
外文摘要：Tomato yellow leaf curl virus (TYLCV) causes great losses in tomato production. In addition to tomato, TYLCV infects many crops or weeds as alternative hosts. These alternative hosts may serve as reservoirs for TYLCV survival and spread. Here, we tested the capability of cultivated, flue-cured tobacco to act as a reservoir host plant for TYLCV. TYLCV DNA was detected in nine flue-cured tobacco cultivars inoculated with an infectious TYLCV clone, although no visible symptoms developed on TYLCV-infected tobacco plants. The percentage of whiteflies with viral DNA increased with an increasing acquisition access period (AAP) and reached 100% after a 12 h AAP on infected tobacco plants. Using infected tobacco plants as virus resources, TYLCV was capable of being transmitted to tobacco and tomato plants by whiteflies, and typical symptoms of TYLCV infection were observed on infected tomato plants but not on infected tobacco plants. Our results suggest that fluecured tobacco can serve as a reservoir host plant for TYLCV and may play an important role in the spread of TYLCV epidemics in China.
作者：Li, Meng; Li, Xiao-Juan; Su, Yun-Lin
作者单位：中国科学院
期刊名称：PLOS ONE
期刊影响因子：2.806
出版年份：2017
出版刊次：12
点击下载：通过农业接种和烟粉虱MED介导传播确定烤烟为番茄黄化曲叶病毒寄主植物的研究