中文摘要：新德里番茄黄化曲叶病毒（ToLCNDV）（双生病毒菜豆金黄花叶病毒属）最近已传入西方地中海国家。西班牙的隔离群构成了一个新的病毒株，将其命名为ToLCNDVES，该病毒可对商业西葫芦和甜瓜的生产造成损失；虽然比较少，但在商业番茄植株上也有检测到该病毒。本研究开发了一个tissue-print杂交测试检测病毒的两种基因组成分以及TaqMan qPCR检测评估植株基因组复制数量。根据本研究获得的相关数据，两种植物病毒滴度的差异有助于解释病毒在症状表达、检测能力及传播方面的不同。
外文摘要：Tomato leaf curl New Delhi virus (ToLCNDV) (family Geminiviridae, genus Begomovirus) has recently been introduced in western Mediterranean countries. Isolates in Spain constitute a new strain, denominated ToLCNDVES, that is causing losses in commercial zucchini and melon crops; however, it is also, although less often, detected in commercial tomato crops. We developed a tissue-print hybridization test to detect the two genomic components of the virus and a TaqMan quantitative polymerase chain reaction (qPCR) test to estimate the number of genome copies in plants. qPCR was approximately 104 to 106 times more sensitive than tissue-print hybridization to detect viral genomic DNA-A and DNA-B, respectively. It also detected the virus in more experimentally and naturally ToLCNDV-ES-infected zucchini squash and tomato plants. ToLCNDV-ES DNA-A titers were significantly lower in tomato than in zucchini plants, often falling below the detection limits in the hybridization test. In addition, the DNA-B accumulation was impaired in tomato when compared with zucchini. According to the data obtained in this study, the differences in viral titers of both plant species contribute to explain the dissimilarities in symptom expression, capability of detection, and transmission of the virus.
作者：Simon, Almudena; Ruiz, Leticia; Velasco, Leonardo; 等
作者单位：西班牙IFAPA Ctr La Mojonera
期刊名称：PLANT DISEASE
期刊影响因子：3.173
出版年份：2018
出版刊次：1
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