中文摘要：葡萄茎痘相关病毒（GRSPaV）是葡萄树上最常见的病毒之一。本研究的目的是对16个巴西GRSPaV分离株外壳蛋白（CP）基因的分子特征进行研究，以确定该病毒是否出现了分子变异型。对9个葡萄树样本进行了评估，并从中提取了dsRNA。通过下一代测序（NGS）生成核苷酸序列。得到了15个GRSPaV CP基因全序列，并对其进行了系统发育分析。序列的多重比对结果显示，核苷酸的一致性为82%至99%，这表明巴西分离株的CPs之间具有高度变异性。本研究揭示了GRSPaV的遗传变异性（含有3个分子变异型）也存在于巴西葡萄树基因型中。
外文摘要：Grapevine rupestris stem pitting-associated virus (GRSPaV) is one of the most common viruses of grapevine. It is involved in the graft-transmissible disease rupestris stem pitting of the rugose wood complex. The objective of the research was to perform the molecular characterization of the coat protein (CP) gene of sixteen Brazilian GRSPaV isolates aiming to determine the occurrence of molecular variants (strains) of this virus. Nine grapevine samples were evaluated, from which dsRNA was extracted. Nucleotide sequences were generated by Next generation sequencing (NGS). Fifteen complete sequences of the GRSPaV CP gene were obtained and phylogenetically analyzed. Multiple alignments of the sequences showed identities of nucleotides ranging from 82% to 99%, suggesting high variability among the CPs of Brazilian isolates. The study revealed that genetic variability of GRSPaV comprising three molecular variants is also present in Brazilian grapevine genotypes.
外文关键词：GRSPaV; Foveavirus; strains; lineages; genetic variability
作者：Martins Fajardo, Thor Vinicius; Kin, Alexis Cardama; Nickel, Osmar
作者单位：巴西农牧科学研究院
期刊名称：CIENCIA RURAL
期刊影响因子：0.417
出版年份：2017
出版刊次：11
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