中文摘要：灰飞虱是最具破坏性的水稻害虫之一。在许多生物学特征如寄主范围、传播能力和植物媒介病毒等方面，灰飞虱都不同于另两种基因组序列已研究公开的水稻飞虱即白背飞虱和褐飞虱。破译灰飞虱的基因组将有助于进一步理解这三种水稻飞虱生物学差异的遗传学基础。研究发现：共产生190 Gb Illumina数据和32.4 Gb Pacbio数据，并用于拼装高质量的灰飞虱基因组序列（长为541 Mb，重叠群N50为118 Kb，scaffold N50为1.08 Mb）。与褐飞虱和白背飞虱相比，灰飞虱的基因组最小，基因数量最少。基因家族的扩增与转录组分析为灰飞虱和其他两种飞虱在寄主范围、迁移习性和植物病毒传播等重要特征方面差异的遗传学基础提供了提示。本研究报道了灰飞虱高质量的基因组拼装，这不仅是进行灰飞虱生物学研究及其与植物寄主和植物病毒之间交互作用研究重要的基因组资源，也是将灰飞虱与其他飞虱进行比较研究的重要资源。
外文摘要：Background: Laodelphax striatellus Fallen (Hemiptera: Delphacidae) is one of the most destructive rice pests. L. striatellus is different from 2 other rice planthoppers with a released genome sequence, Sogatella furcifera and Nilaparvata lugens, inmany biological characteristics, such as host range, dispersal capacity, and vectoring plant viruses. Deciphering the genome of L. striatellus will further the understanding of the genetic basis of the biological differences among the 3 rice planthoppers. Findings: A total of 190 Gb of Illumina data and 32.4 Gb of Pacbio data were generated and used to assemble a high-quality L. striatellus genome sequence, which is 541 Mb in length and has a contig N50 of 118 Kb and a scaffold N50 of 1.08 Mb. Annotated repetitive elements account for 25.7% of the genome. A total of 17 736 protein-coding genes were annotated, capturing 97.6% and 98% of the BUSCO eukaryote and arthropoda genes, respectively. Compared with N. lugens and S. furcifera, L. striatellus has the smallest genome and the lowest gene number. Gene family expansion and transcriptomic analyses provided hints to the genomic basis of the differences in important traits such as host range, migratory habit, and plant virus transmission between L. striatellus and the other 2 planthoppers. Conclusions: We report a high-quality genome assembly of L. striatellus, which is an important genomic resource not only for the study of the biology of L. striatellus and its interactions with plant hosts and plant viruses, but also for comparison with other planthoppers.
外文关键词：comparative genomics; insects; genome sequencing; annotation; virus transmission
作者：Zhu, Junjie; Jiang, Feng; Wang, Xianhui; 等
作者单位：中国科学院
期刊名称：GIGASCIENCE
期刊影响因子：6.871
出版年份：2017
出版刊次：11
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